NaCl and CuSO4 treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L
NaCl and CuSO4 treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L
复制标题
DOI：
10.1046/j.1365-3040.1999.00404.x
复制
发表时间：
1999-04
影响因子：
7.3
通讯作者：
A. Savouré;D. Thorin;M. Davey;X. Hua;S. Mauro;M. Montagu;D. Inzé;N. Verbruggen
中科院分区：
文献类型：
--
作者：
A. Savouré;D. Thorin;M. Davey;X. Hua;S. Mauro;M. Montagu;D. Inzé;N. Verbruggen
为了更好地了解NaCl胁迫产生的氧化胁迫，我们比较了毒性亚致死浓度的NaCl（150 mM）和CuSO4（100 μM）对烟草抗氧化防御反应的影响。在水培中生长的植物。短期和长期的适应性反应进行了研究，以及根切割的影响，使离子的快速吸收。我们的研究结果表明，光合作用已经受到影响后12小时的两种治疗和诱导光抑制。此外，这两种处理显示出不同的抗氧化系统基因的诱导模式。主要是，NaCl刺激过氧化氢酶活性通过激活的Cat2和Cat3基因，而与硫酸铜，转录水平，蛋白质水平，谷胱甘肽过氧化物酶，抗坏血酸过氧化物酶和超氧化物歧化酶的酶活性差异诱导。抗氧化剂抗坏血酸和谷胱甘肽被发现在12小时后被大量氧化，这表明回收过程无法科普压力。此外，NaCl和CuSO_4处理刺激的渗透压脯氨酸的积累。因此，我们得出结论，不同类型的离子的吸收和积累导致产生不同的抗氧化反应，在N。白花丹
To gain better insights into the generation of oxidative stress by NaCl stress, we compared the effects of toxic, sublethal concentrations of NaCl (150 mM) with those of CuSO4 (100 μM) on the antioxidant defence responses of Nicotiana plumbaginifolia L. plants grown in hydroponic culture. Short- and long-term adaptive responses were investigated as well as the effects of root cutting to allow rapid absorption of ions. Our results show that photosynthesis is already affected after 12 h of both treatments and that photoinhibition is induced. Furthermore, the two treatments showed distinct patterns of induction of the genes of the antioxidant systems. Principally, NaCl stimulated catalase activity through activation of the Cat2 and Cat3 genes, whereas with CuSO4, transcript levels, protein levels, and enzymatic activities of glutathione peroxidase, ascorbate peroxidase, and superoxide dismutase were differentially induced. The antioxidants ascorbate and glutathione were found to be largely oxidized after 12 h, suggesting an inability of the recycling process to cope with the stress. In addition, both NaCl and CuSO4 treatments stimulated the accumulation of the osmolyte proline. Therefore, we conclude that the uptake and accumulation of different types of ions lead to the generation of distinct antioxidant responses in N. plumbaginifolia.