JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates
JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates
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DOI：
10.1093/embo-reports/kvf008
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发表时间：
2002-01-01
期刊：
影响因子：
7.7
通讯作者：
Nishida, E
中科院分区：
文献类型：
--
作者：
Yamanaka, H;Moriguchi, T;Nishida, E
最近在果蝇中的遗传学研究确定了一种新的非经典Wnt途径，平面细胞极性（PCP）途径，通过JNK信号控制果蝇上皮细胞极性。最近，在脊椎动物胚胎原肠胚形成过程中调节会聚伸展运动的途径已被证明是脊椎动物PCP途径的等价物。然而，目前尚不清楚JNK通路是否在这种非经典Wnt通路中起作用，以调节脊椎动物的会聚伸展运动。此外，尚不清楚JNK是否实际上被Wnt刺激激活。在这里，我们表明，Wnt 5a是能够激活JNK在培养的细胞，并提出证据表明，JNK途径介导的Wnt 5a的行动，以调节收敛的延伸运动在非洲爪蟾。因此，我们的研究结果表明，非经典的Wnt/JNK途径是保守的脊椎动物和无脊椎动物，并确定JNK具有活性，以调节形态发生细胞的运动。
Recent genetic studies in Drosophila identified a novel non-canonical Wnt pathway, the planar cell polarity (PCP) pathway, that signals via JNK to control epithelial cell polarity in Drosophila. Most recently, a pathway regulating convergent extension movements during gastrulation in vertebrate embryos has been shown to be a vertebrate equivalent of the PCP pathway. However, it is not known whether the JNK pathway functions in this non-canonical Wnt pathway to regulate convergent extension movements in vertebrates. In addition, it is not known whether JNK is in fact activated by Wnt stimulation. Here we show that Wnt5a is capable of activating JNK in cultured cells, and present evidence that the JNK pathway mediates the action of Wnt5a to regulate convergent extension movements in Xenopus. Our results thus demonstrate that the non-canonical Wnt/JNK pathway is conserved in both vertebrate and invertebrate and define that JNK has an activity to regulate morphogenetic cell movements.