Apoptosis of Acanthamoeba castellanii Trophozoites Induced by Oleic Acid
Apoptosis of Acanthamoeba castellanii Trophozoites Induced by Oleic Acid
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油酸诱导卡氏棘阿米巴滋养体细胞凋亡。
DOI：
10.1111/jeu.12454
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发表时间：
2018-03-01
影响因子：
2.2
通讯作者：
Cheng, Xunjia
中科院分区：
文献类型：
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作者：
Wu, Duo;Qiao, Ke;Cheng, Xunjia
阿米巴属在某些情况下可以寄生，引起严重的人类感染，包括阿米巴角膜炎、肉芽肿性阿米巴脑炎和皮肤棘阿米巴病。分析了卡氏阿米巴滋养体的脂肪酸组成，并对主要脂肪酸油酸和花生四烯酸进行了体外抑菌活性测定。油酸显著抑制卡氏按蚊的生长，浓度为200 M时滋养体存活率为57.4%。Caspase-3染色和Annexin V检测结果显示卡氏不动杆菌滋养体发生了凋亡。定量PCR和斑点印迹分析显示后半胱天冬酶和白细胞介素-1转化酶的水平增加，这也是细胞凋亡的指示。相比之下，花生四烯酸对卡氏不动杆菌滋养体生长的抑制作用可忽略不计。Atg 3、Atg 8和LC 3A/B基因的刺激表达和单丹酰尸胺标记表明，油酸通过触发滋养体的自噬来诱导细胞凋亡。
Acanthamoeba spp. can be parasitic in certainsituations and are responsible for serious human infections, including Acanthamoeba keratitis, granulomatous amoebic encephalitis, and cutaneous acanthamoebiasis. We analyzed the fatty acid composition of Acanthamoeba castellanii trophozoites and tested the inhibitory activity of the main fatty acids, oleic acid and arachidonic acid, invitro. Oleic acid markedly inhibited the growth of A.castellanii, with trophozoite viability of 57.4% at a concentration of 200M. Caspase-3 staining and annexin V assays showed that apoptotic death occurred in A.castellanii trophozoites. Quantitative PCR and dot blot analysis showed increased levels of metacaspase and interleukin-1 converting enzyme, which is also an indication of apoptosis. In contrast, arachidonic acid showed negligible inhibition of growth of A.castellanii trophozoites. Stimulated expression of Atg3, Atg8 and LC3A/B genes and monodansylcadaverine labeling suggested that oleic acid induces apoptosis by triggering autophagy of trophozoites.