Simultaneous Multi-plane Imaging of Neural Circuits.
Simultaneous Multi-plane Imaging of Neural Circuits.
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DOI：
10.1016/j.neuron.2015.12.012
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发表时间：
2016-01-20
期刊：
影响因子：
16.2
通讯作者：
Peterka DS
中科院分区：
文献类型：
--
作者：
Yang W;Miller JE;Carrillo-Reid L;Pnevmatikakis E;Paninski L;Yuste R;Peterka DS
记录大量神经元的活动是了解神经回路突现功能的重要一步。在这里，我们提出了一种简单的全息方法，可以在体内同时对小鼠大脑皮质多个区域和多层的神经元群体进行双光子钙成像。我们使用神经元位置的先验知识、活动稀疏性和有约束的非负矩阵分解算法来从同时成像的位于不同焦平面或视场的神经元中提取信号。我们的激光多路复用方法简单、快速，可以作为一种通用方法来成像大脑中多个区域的三维神经回路的活动。
Recording the activity of large populations of neurons is an important step towards understanding the emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in vivo. We use prior knowledge of neuronal locations, activity sparsity and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain.