Cellular uptake of magnetic nanoparticles imaged and quantified by magnetic particle imaging
Cellular uptake of magnetic nanoparticles imaged and quantified by magnetic particle imaging
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DOI：
10.1038/s41598-020-58853-3
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发表时间：
2020-02-05
影响因子：
4.6
通讯作者：
Wiekhorst, Frank
中科院分区：
文献类型：
--
作者：
Paysen, Hendrik;Loewa, Norbert;Wiekhorst, Frank
磁粒子成像(MPI)是一种非侵入性、非电离成像技术，用于显示和定量磁性纳米颗粒(MNPs)。该技术特别适用于细胞成像，因为它提供了来自周围组织的零背景贡献，高灵敏度，以及良好的空间和时间分辨率。先前的研究表明，在细胞结合和内化过程中，MNPs的动态磁性行为会发生变化。在这项研究中，我们演示了这种信息是如何在MPI成像信号中编码的。通过MPI成像，我们能够在重建图像中区分游离和细胞结合的MNPs。这项技术被用来成像和量化在体外发生的变化，当游离的MNPs与细胞接触并随着时间的推移经历细胞摄取时。定量的MPI结果通过铁含量的比色测量得到了验证。结果表明，MPI结果与细胞结合MNPs定量参考方法的平均相对偏差为23.8%。利用这项技术，细胞对MNPs的摄取可以直接从第一次接触MNP的细胞中成像和量化，从而提供关于细胞摄取动态的信息。
Magnetic particle imaging (MPI) is a non-invasive, non-ionizing imaging technique for the visualization and quantification of magnetic nanoparticles (MNPs). The technique is especially suitable for cell imaging as it offers zero background contribution from the surrounding tissue, high sensitivity, and good spatial and temporal resolutions. Previous studies have demonstrated that the dynamic magnetic behaviour of MNPs changes during cellular binding and internalization. In this study, we demonstrate how this information is encoded in the MPI imaging signal. Through MPI imaging we are able to discriminate between free and cell-bound MNPs in reconstructed images. This technique was used to image and quantify the changes that occur in-vitro when free MNPs come into contact with cells and undergo cellular-uptake over time. The quantitative MPI results were verified by colorimetric measurements of the iron content. The results showed a mean relative difference between the MPI results and the reference method of 23.8% for the quantification of cell-bound MNPs. With this technique, the uptake of MNPs in cells can be imaged and quantified directly from the first MNP cell contact, providing information on the dynamics of cellular uptake.