Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph
Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph
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DOI：
10.1016/j.molbiopara.2009.10.002
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发表时间：
2010-02-01
影响因子：
1.5
通讯作者：
Simoes, Nelson
中科院分区：
文献类型：
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作者：
Hao, You-Jin;Montiel, Rafael;Simoes, Nelson
斯氏线虫（Steinernema carpocapsae）是一种广泛用于害虫防治的昆虫寄生线虫。已发现该线虫控制昆虫的功效与感染阶段的致病性有关。为了研究该寄生虫的寄生机制，构建了诱导的S。carpocapsae寄生相产生。共对2500个克隆进行测序，获得2180条高质量的EST。聚类分析共产生1592个独特的序列，包括1393个单例。约63%的独特序列具有显著的命中（例如，
Steinernema carpocapsae is an insect parasitic nematode widely used in pest control programs. The efficacy of this nematode in controlling insects has been found to be related to the pathogenicity of the infective stage. In order to study the parasitic mechanisms exhibited by this parasite, a cDNA library of the induced S. carpocapsae parasitic phase was generated. A total of 2500 clones were sequenced and 2180 high-quality ESTs were obtained from this library. Cluster analysis generated a total of 1592 unique sequences including 1393 singletons. About 63% of the unique sequences had significant hits (e