Repair of orbital wall defects using biocoral scaffolds combined with bone marrow stem cells enhanced by human bone morphogenetic protein-2 in a canine model.
Repair of orbital wall defects using biocoral scaffolds combined with bone marrow stem cells enhanced by human bone morphogenetic protein-2 in a canine model.
复制标题
DOI：
10.3892/ijmm_00000494
复制
发表时间：
2010-10
影响因子：
5.4
通讯作者：
Caiwen Xiao;Huifang Zhou;S. Ge;Ting‐ting Tang;Hongliang Hou;Min Luo;Xianqun Fan
中科院分区：
文献类型：
--
作者：
Caiwen Xiao;Huifang Zhou;S. Ge;Ting‐ting Tang;Hongliang Hou;Min Luo;Xianqun Fan
外伤、感染或肿瘤引起的眶骨缺损的修复一直是重建外科的挑战。应用组织工程技术修复大型动物模型眼眶骨缺损的研究较少。因此，我们研究了人骨形态发生蛋白-2（BMP-2）增强的组织工程骨对犬眼眶壁缺损修复的影响。从16只Beagle犬体内分离培养自体骨髓基质细胞（BMSCs）。第2代细胞转染含人BMP 2的腺病毒（adv-BMP 2），并使用BMP 2表达的BMSCs接种在生物珊瑚支架上构建组织工程骨。在犬眶内侧壁双侧创建圆形缺损（直径12 mm），采用以下方法之一进行治疗：adv-BMP 2转染的BMSC/珊瑚复合材料（第I组，n=8）、BMSC/珊瑚复合材料（第II组，n=8）、单独的生物珊瑚（第III组，n=8），或不进行治疗（第IV组，n=8）。分别于术后12周和24周采集每组4个样本，通过显微计算机断层扫描（micro-CT）测量确定新再生骨的体积和密度。四环素/钙黄绿素标记和组织形态计量学分析测量新骨沉积和再生率。结果表明，犬眼眶12 mm圆形缺损为临界大小缺损，显微CT和组织形态计量学检测结果表明，BMSCs和BMP 2联合递送组（I组）对眼眶骨缺损的再生效果最好，而其他组无BMP 2。Biocoral支架复合BMP-2增强的BMSCs可促进犬眶内壁临界缺损的愈合。
Repair of orbital bone defects caused by trauma, infection or cancer is a continuous challenge in reconstructive surgery. Few studies have reported the application of tissue engineering for the repair of orbital bone defects in large animal models. Thus, we investigated the effects of tissue-engineered bone enhanced by the human bone morphogenetic protein-2 (BMP2) on the repair of orbital wall defects in a canine model. Autologous bone marrow stromal cells (BMSCs) from 16 Beagle dogs were isolated and cultured in vitro. Passage 2 cells were transfected with adenovirus containing human BMP2 (adv-BMP2) and tissue-engineered bone was constructed using BMP2-expressing BMSCs seeded on a biocoral scaffold. Circular defects (12-mm diameter) created bilaterally in the canine medial orbital wall, were treated with one of the following: adv-BMP2-transfected BMSC/coral composite (group I, n=8), BMSC/coral composite (group II, n=8), biocoral alone (group III, n=8), or were left untreated (group IV, n=8). Four samples from each group were harvested at 12 and 24 weeks after surgery, and the volume and density of newly regenerated bone were determined by micro-computed tomographic (micro-CT) measurement. The rate of new bone deposition and regeneration was measured by tetracycline/calcein labeling and histomorphometric analysis. The results showed that a canine 12-mm circular orbital defect was a critical-sized defect, and the micro-CT and histomorphometry detection results indicated that the combined delivery of BMSCs and BMP2 (group I) resulted in the highest regenerative effects on orbital bone defects, compared to the other groups without BMP2. Biocoral scaffolds combined with BMSCs enhanced by BMP2 could improve the healing of critical-sized medial orbital wall defects in canines.