Evolution in Pseudomonas fluorescens.
Evolution in Pseudomonas fluorescens.
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荧光假单胞菌的进化。
DOI：
10.1099/00221287-120-2-485
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Doudoroff,M
中科院分区：
文献类型：
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作者：
Champion,AB;Barrett,EL;Palleroni,NJ;Soderberg,KL;Kunisawa,R;Contopoulou,R;Wilson,AC;Doudoroff,M
本文对93株荧光假单胞菌间的关系进行了研究：(1)对150份表型试验结果进行了数值分类分析，(2)对16株参考菌株进行了DNA杂交研究，(3)对6株针对azurin蛋白抗体的参考菌株进行了定量微补体固定研究。总的来说，这些菌株属于不同的集群。基于azurin免疫相似性对这些簇的分配与基于DNA同源性的分配有98%的一致性，这表明许多基因将遵循相同的模式。根据基因型（DNA, azurin）聚集的菌株中，88%的菌株也根据表型聚集。偶尔观察到的基因型和表型数据之间的不一致可能是由于表型进化的可变速率。这些结果提供了一个视角的作用水平和垂直转移的基因在这一细菌群的进化。
The relationships among 93 strains ofPseudomonas fluorescenswere investigated by (1) a numerical taxonomic analysis on the results of 150 phenotypic tests, (2) DNA hybridization studies using 16 reference strains, (3) quantitative microcomplement fixation studies using six reference strains with antibodies directed against the protein azurin. In general, the strains fell into distinct clusters. Assignment to these clusters on the basis of azurin immunological similarity showed 98% agreement with assignment based on DNA homology, suggesting that many genes will follow the same pattern. Of the strains that clustered on the basis of genotype (DNA, azurin) 88% also clustered on the basis of phenotype. The occasional non-congruency observed between the genotypic and phenotypic data may be due to the variable rates of phenotypic evolution. These results provide a perspective on the roles of horizontal and vertical transfer of genes in the evolution of this bacterial group.