Spacer sequences downstream of the 28S RNA coding region are part of the mouse rDNA transcription unit.
Spacer sequences downstream of the 28S RNA coding region are part of the mouse rDNA transcription unit.
复制标题
28S RNA 编码区下游的间隔序列是小鼠 rDNA 转录单位的一部分。
DOI：
10.1093/nar/13.7.2293
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发表时间：
1985
影响因子：
14.9
通讯作者：
E. Roth
中科院分区：
文献类型：
--
作者：
I. Grummt;H. Sorbaz;A. Hofmann;E. Roth
有证据表明，超过300 bp的间隔序列下游的28S RNA编码序列的小鼠rDNA转录单位的一部分。在两种无细胞转录系统中的研究以及细胞RNA的分析表明，RNA聚合酶I不在所用rDNA克隆中所含的334 bp 3'末端间隔区序列内终止。定量杂交数据、S1作图实验和核RNA的北方分析表明，14 kb的前rRNA分子与28 S和3' NTS特异性DNA探针具有相同的杂交效率。这表明rRNA前体在5'和3'端都含有数百个碱基的外部转录间隔区序列，这些序列在随后的加工反应中被消除。
Evidence is presented that more than 300 bp of spacer sequences downstream of the 28S RNA coding sequence are part of the mouse rDNA transcription unit. Studies in two cell-free transcription systems as well as analysis of cellular RNA indicate that RNA polymerase I does not terminate within the 334 bp 3' terminal spacer sequences contained in the rDNA clone used. Quantitative hybridization data, S1 mapping experiments and Northern analysis of nuclear RNA showed that the 14 kb pre-rRNA molecules hybridize with the same efficiency to both the 28S and the 3' NTS specific DNA probe. This indicates that the rRNA precursor contains both at the 5' and 3' end several hundreds bases of external transcribed spacer sequences which are eliminated in subsequent processing reactions.