Proteomic and transcriptional analysis of interaction between oral microbiota Porphyromonas gingivalis and Streptococcus oralis.
Proteomic and transcriptional analysis of interaction between oral microbiota Porphyromonas gingivalis and Streptococcus oralis.
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DOI：
10.1021/pr500848e
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发表时间：
2015-01
影响因子：
4.4
通讯作者：
K. Maeda;H. Nagata;M. Ojima;A. Amano
中科院分区：
文献类型：
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作者：
K. Maeda;H. Nagata;M. Ojima;A. Amano
牙龈卟啉单胞菌是一种主要的牙周病原体，与链球菌等其他口腔细菌形成生物膜。在这里，通过使用鸟枪蛋白质组学，我们研究了牙龈卟啉单胞菌和口腔链球菌形成混合生物膜的分子基础。我们在生物膜中总共鉴定出了 593 种细菌蛋白。与牙龈卟啉单胞菌单生物膜中的表达谱相比，混合生物膜中 3 种蛋白质的表达被诱导，31 种蛋白质的表达受到抑制。此外，与其单型特征相比，混合生物膜中两种口链球菌蛋白的表达增加，而两种蛋白的表达减少。使用定量逆转录聚合酶链式反应对选定基因的 mRNA 表达分析证实了蛋白质组学数据，其中包括与生物膜相关的牙龈卟啉单胞菌 FimA 和口链球菌甘油醛-3-磷酸脱氢酶的过度表达。结果还表明，口链球菌调节牙龈卟啉单胞菌luxS的转录活性，以影响自诱导剂2依赖性信号传导。这些发现表明，几种功能分子参与牙龈卟啉单胞菌和口腔链球菌之间生物膜的形成。
Porphyromonas gingivalis, a major periodontal pathogen, forms biofilm with other oral bacteria such as streptococci. Here, by using shotgun proteomics, we examined the molecular basis of mixed-biofilm formation by P. gingivalis with Streptococcus oralis. We identified a total of 593 bacterial proteins in the biofilm. Compared to the expression profile in the P. gingivalis monobiofilm, the expression of three proteins was induced and that of 31 proteins was suppressed in the mixed biofilm. Additionally, the expression of two S. oralis proteins was increased, while that of two proteins was decreased in the mixed biofilm, as compared to its monotypic profile. mRNA expression analysis of selected genes using a quantitative reverse transcription polymerase chain reaction confirmed the proteomics data, which included overexpression of P. gingivalis FimA and S. oralis glyceraldehyde-3-phosphate dehydrogenase in association with the biofilm. The results also indicated that S. oralis regulates the transcriptional activity of P. gingivalis luxS to influence autoinducer-2-dependent signaling. These findings suggest that several functional molecules are involved in biofilm formation between P. gingivalis and S. oralis.