Development, Characterization, and Application of Monoclonal Antibodies against Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein
Development, Characterization, and Application of Monoclonal Antibodies against Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein
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DOI：
10.1128/cvi.00293-10
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发表时间：
2010-12-01
影响因子：
--
通讯作者：
Suresh, Mavanur R.
中科院分区：
文献类型：
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作者：
Das, Dipankar;Kammila, Sriram;Suresh, Mavanur R.
应用杂交瘤技术制备了5株抗SARS冠状病毒重组核衣壳蛋白（NP）的单克隆抗体。蛋白质印迹法的表位定位显示这些抗SARS-CoV NP单抗结合NP的不同结构域。这些抗SARS-CoV NP单克隆抗体具有高度的特异性，是开发SARS-CoV早期和敏感诊断方法的理想候选物。
Five monoclonal antibodies (MAbs) against recombinant nucleocapsid protein (NP) of severe acute respiratory syndrome (SARS)-causing coronavirus (CoV) were developed by hybridoma technology. Epitope mapping by Western blotting showed that these anti-SARS-CoV NP MAbs bind to distinct domains of NP. These anti-SARS-CoV NP MAbs, with their high specificity, are potentially ideal candidates for developing early and sensitive diagnostic assays for SARS-CoV.