Chicken microsatellite primers are not efficient markers for Japanese quail
Chicken microsatellite primers are not efficient markers for Japanese quail
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DOI：
10.1046/j.1365-2052.2001.00699.x
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发表时间：
2001-02-01
期刊：
影响因子：
2.4
通讯作者：
Ito, S
中科院分区：
文献类型：
--
作者：
Inoue-Murayama, M;Kayang, BB;Ito, S
家禽或鸡（Gallus Gallus）和日本鹌鹑（Coturnix japonica）属于雉科。鸡和鹌鹑之间的标记信息交换是构建高分辨率比较遗传图谱的重要一步，其中包括几种重要的农业家禽物种。我们测试了鸡微卫星标记，看看它们是否适合作为日本鹌鹑的遗传连锁标记。26%（31/120）的鸡引物扩增到了日本鹌鹑的单个位点，65%（20/31）的引物扩增到了日本鹌鹑的多态性位点。11个多态位点由于在某些个体中缺乏扩增或非特异性扩增频率高而被排除在外。其余9个位点的序列信息显示，其中6个位点含有微卫星，这些微卫星与鸡的同源区域几乎相同。对于这6个基因座，在50只不相关的鹌鹑中估计了等位基因频率。虽然在鹌鹑中工作良好的极少数鸡标记可以作为比较定位的锚点，但大多数鸡标记对鹌鹑的研究没有用处。因此，更多的努力应该致力于开发鹌鹑特异性标记，而不是试图使鸡标记适应鹌鹑的工作。
Domestic fowl or chicken (Gallus gallus) and Japanese quail (Coturnix japonica) belong to the family Phasianidae. The exchange of marker information between chicken and quail is an important step towards the construction of a high-resolution comparative genetic map in Phasianidae, which includes several poultry species of agricultural importance. We tested chicken microsatellite markers to see if they would be suitable as genetic linkage markers in Japanese quail. Twenty-six per cent (31/120) of chicken primers amplified individual loci in Japanese quail and 65% (20/31) of the amplified loci were found to be polymorphic. Eleven of the polymorphic loci were excluded as uninformative because of the lack of amplification in some individuals or high frequency of nonspecific amplification. The sequence information of the remaining nine loci revealed six of them to contain microsatellites that were nearly identical with those of the orthologous regions in chicken. For these six loci, allele frequencies were estimated in 50 unrelated quails. Although the very few chicken markers that do work well in quail could be used as anchor points for a comparative mapping, most chicken markers are not useful for studies in quail. Therefore, more effort should be committed to developing quail-specific markers rather than attempting to adapt chicken markers for work in quail.