Dual fluorescence sensor for trace oxygen and temperature with unmatched range and sensitivity
Dual fluorescence sensor for trace oxygen and temperature with unmatched range and sensitivity
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DOI：
10.1021/ac801034p
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发表时间：
2008-08-15
影响因子：
7.4
通讯作者：
Wolfbeis, Otto S.
中科院分区：
文献类型：
--
作者：
Baleizao, Carlos;Nagl, Stefan;Wolfbeis, Otto S.
介绍了一种氧、温度双传感器，该传感器具有氧灵敏度高、温度范围宽的特点。双重传感是基于发光寿命测量的。该新型传感器包含两种结合在聚合物薄膜中的发光化合物。这种温度敏感的染料(Ru-三-1，10-菲咯啉)具有高度的温度依赖性发光，并被加入到聚丙烯腈中以避免对氧的交叉敏感。富勒烯C-70在高温下具有强烈的热激活延迟荧光，对氧非常敏感，因此被用作氧敏感探针。利用温度传感器计算了C-70对温度的交叉敏感性。C-70被结合到一种高度透氧的聚合物中，要么是乙基纤维素，要么是有机硅。这两种发光探针具有不同的发射光谱和衰减时间，使用这两个参数可以区分它们的发射。空间分辨传感是通过荧光寿命成像实现的。传感器对氧气的响应时间较短。该双传感器的工作温度范围至少在0摄氏度到120摄氏度之间，对氧的检测极限在ppbv范围内，在氧浓度高达至少50ppmv的情况下工作。这些传感器的性能超过了迄今报道的所有双氧传感器和温度传感器。本研究中提出的双传感器特别适用于高温和超低氧水平等极端条件下的测量。这种双传感器是在许多科学或商业重要应用方面向前迈出的关键一步，包括食品包装、监测高温微生物、空间技术以及在检测氧气泄漏方面的安全和安保应用。
An optical dual sensor for oxygen and temperature is presented that is highly oxygen sensitive and covers a broad temperature range. Dual sensing is based on luminescence lifetime measurements. The novel sensor contains two luminescent compounds incorporated into polymer films. The temperature-sensitive dye (ruthenium tris-1,10-phenanthroline) has a highly temperature-dependent luminescence and is incorporated in poly(acrylonitrile) to avoid cross-sensitivity to oxygen. Fullerene C-70 was used as the oxygen-sensitive probe owing to its strong thermally activated delayed fluorescence at elevated temperatures that is extremely oxygen sensitive. The cross-sensitivity of C-70 to temperature is accounted for by means of the temperature sensor. C-70 is incorporated into a highly oxygen-permeable polymer, either ethyl cellulose or organosilica. The two luminescent probes have different emission spectra and decay times, and their emissions can be discriminated using both parameters. Spatially resolved sensing is achieved by means of fluorescence lifetime imaging. The response times of the sensor to oxygen are short. The dual sensor exhibits a temperature operation range between at least 0 and 120 degrees C, and detection limits for oxygen in the ppbv range, operating for oxygen concentrations up to at least 50 ppmv. These ranges outperform all dual oxygen and temperature sensors reported so far. The dual sensor presented in this study is especially appropriate for, measurements under extreme conditions such as high temperatures and ultralow oxygen levels. This dual sensor is a key step forward in a number of scientifically or commercially important applications including food packaging, for monitoring of hyperthermophilic microorganisms, in space technology, and safety and security applications in terms of detection of oxygen leaks.