Cloning and nucleotide sequences of the genes involved in the meta-cleavage pathway of cumene degradation in Pseudomonas fluorescens IP01
Cloning and nucleotide sequences of the genes involved in the meta-cleavage pathway of cumene degradation in Pseudomonas fluorescens IP01
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DOI：
10.1016/0922-338x(96)82216-1
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Omori, T
中科院分区：
文献类型：
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作者：
Habe, H;Kimura, T;Omori, T
从荧光假单胞菌IP 01中克隆了一个SalI片段，该片段含有编码将枯烯（异丙苯）降解为2-羟基-6-氧代-7-甲基辛-2，4-二烯酸的酶的基因簇的下游区域。我们测定了4，045-bp区域的核苷酸序列，并鉴定了三个开放阅读框架（ORF）。比较由三个ORF编码的氨基酸序列与由bph、dmp、xyl和tod操纵子编码的酶的氨基酸序列表明，ORF编码在间位裂解途径中将2-羟基戊-2，4-二烯酸转化为克雷布斯循环中间体所需的酶。与先前表征的cumA 1A 2（ORF 3）A3 A4 BC基因一起，已经鉴定了10个参与枯烯降解的基因。
A SalI fragment which contained the downstream region of a gene cluster encoding enzymes degrading cumene (isopropylbenzene) to 2-hydroxy-6-oxo-7-methylocta-2,4-dienoic acid was cloned from Pseudomonas fluorescens IP01. We determined the nucleotide sequence of the 4,045-bp region, and identified three open reading frames (ORFs). Comparison of amino acid sequences encoded by the three ORFs with those of enzymes encoded by the bph, dmp, xyl and tod operons suggested that the ORFs encode enzymes which are required for conversion of 2-hydroxypenta-2,4-dienoic acid to Krebs cycle intermediates in the meta-cleavage pathway. Taken together with the previously characterized cumA1A2(ORF3)A3A4BC genes, ten genes involved in cumene degradation have been identified.