New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes
New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes
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DOI：
10.1016/j.dci.2017.06.018
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发表时间：
2017-11-01
影响因子：
2.9
通讯作者：
Romero, Alex
中科院分区：
文献类型：
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作者：
Gonzalez-Stegmaier, Roxana;Villarroel-Espindola, Franz;Romero, Alex
神经肽Y （NPY）在哺乳动物中发挥着不同的作用，如：调节食物摄入、记忆保持、心血管功能和焦虑。它也显示在趋化性，T淋巴细胞分化和白细胞迁移的调节。在鱼类中，NPY的表达和功能已被研究，但其免疫调节作用仍未被描述。本研究证实了NPY在炎症条件下在S. solar中的表达和合成，并验证了一种用于硬骨鱼检测NPY的商业抗体。此外，本研究还对NPY在体外和体内的免疫调节作用进行了研究。在合成肽的刺激下，白细胞和SHK细胞的吞噬和超氧阴离子的产生被诱导。体内NPY刺激后，IL-8 mRNA在脾脏、头肾和离体细胞中被选择性强烈诱导。综上所述，NPY在硬骨鱼的免疫组织中表达并调节免疫反应。(C) 2017 Elsevier Ltd.版权所有。
Neuropeptide Y (NPY) plays different roles in mammals such as: regulate food intake, memory retention, cardiovascular functions, and anxiety. It has also been shown in the modulation of chemotaxis, T lymphocyte differentiation, and leukocyte migration. In fish, NPY expression and functions have been studied but its immunomodulatory role remains undescribed. This study confirmed the expression and synthesis of NPY in S. solar under inflammation, and validated a commercial antibody for NPY detection in teleost Additionally, immunomodulatory effects of NPY were assayed in vitro and in vivo. Phagocytosis and superoxide anion production in leukocytes and SHK cells were induced under stimulation with a synthetic peptide. IL-8 mRNA was selectively and strongly induced in the spleen, head kidney, and isolated cells, after in vivo challenge with NPY. All together suggest that NPY is expressed in immune tissues and modulates the immune response in teleost fish. (C) 2017 Elsevier Ltd. All rights reserved.