The Effect of Dynamic Loading on Bacterial Colonization of the Dental Implant Fixture-Abutment Interface: An In Vitro Study
The Effect of Dynamic Loading on Bacterial Colonization of the Dental Implant Fixture-Abutment Interface: An In Vitro Study
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DOI：
10.1563/aaid-joi-d-11-00207
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发表时间：
2014-08-01
影响因子：
1.6
通讯作者：
Wallet, Shannon
中科院分区：
文献类型：
--
作者：
Koutouzis, Theofilos;Mesia, Ruben;Wallet, Shannon
细菌定植在固定-基台界面（FAI）微间隙可能会增加边缘骨质流失。负载对细菌定植的贡献尚未通过体外实验进行彻底评估。本研究的目的是评估动态载荷对口腔微生物在具有莫尔斯-锥度内连接的种植体FAI微间隙内定植的影响。40个种植体根据动态加载条件分为两组（n = 20/组）。组1和组2均由连接到标准基台的固定装置组成，并允许在大肠杆菌细菌溶液中孵育。第二组用咀嚼模拟器加载50万次50 N。断开固定装置和基台后，从基台的螺纹部分提取微生物样本，在适当的条件下进行电镀和培养。第1组20个种植体中有1个，第2组20个种植体中有4个存在大肠杆菌定殖的FAI微间隙。鉴于本研究的局限性，它表明具有内部莫尔斯-锥度连接的植入物在FAI的螺纹部分表现出最小的细菌渗透，并且动态载荷增加了这种细菌渗透的可能性。
Bacterial colonization of the fixture-abutment interface (FAI) microgap may contribute to increased marginal bone loss. The contribution of loading on bacterial colonization has not been thoroughly evaluated with in vitro experiments. The aim of this study was to evaluate the effect of dynamic loading on the colonization of oral microorganisms in the FAI microgap of dental implants with internal Morse-taper connection. Forty implants were divided into two groups (n = 20/group) based on subjection to dynamic loading conditions. Both Group 1 and 2 were comprised of fixtures that connected to standard abutments and allowed to incubate in a bacterial solution of Escherichia coli. The specimens of Group 2 were loaded with 500 000 cycles of 50 N using a chewing simulator. Following disconnection of fixtures and abutments, microbial samples were taken from the threaded portion of the abutment, plated and cultured under appropriate conditions. One of the 20 implants of Group 1 and 4 of the 20 implants of Group 2 had FAI microgaps colonized by E coli. With the limits of this study, it indicates that implants with internal Morse-taper connection exhibited minimal bacterial penetration down to the threaded part of the FAI and that dynamic loading increases the potential for such bacterial penetration.