Age dependence of in situ termostability of collagen in human bone.
Age dependence of in situ termostability of collagen in human bone.
复制标题
人骨中胶原蛋白原位热稳定性的年龄依赖性。
DOI：
10.1007/s00223-002-1065-y
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Wang,X
中科院分区：
文献类型：
--
作者：
Li,X;Agrawal,CM;Wang,X
The hypothesis of this study is that the in situ thermostability of collagen in bone changes with age and such changes relate to the structural properties of collagen and/or the interaction between the collagen and mineral phases. To test the hypothesis, the effect of age on the in situ thermostability of collagen in human bone and its correlation with the mineral and collagen phases were investigated. In this study, 30 human cadaveric femurs were collected and divided into three age groups: young adults (20–45 years), middle aged (46–69 years), and elderly (over 70 years of age). The in situ thermostability of collagen was assessed using high performance liquid chromatography (HPLC) in terms of the amount of heat-induced collagen denaturation at eight temperatures between 25°C and 140°C. In addition to the density and weight fraction of the mineral and organic phases, the concentration of collagen crosslinks was measured to assess the structural integrity of collagen. The results of this study indicate that the in situ thermostability of collagen increases with increasing age, and such age-related changes correlate with the following: collagen molecular structure, amount of noncalcified collagen, and the fraction and density of the mineral phase in bone. These results suggest that the age-related changes in the in situ thermostability of collagen most likely relate to the collagen structure and its interaction with the mineral phase. In addition, the bone remodeling process may play a role in the age-related changes in collagen thermostability because noncalcified collagen is commonly associated with this process.
