Boron deprivation immediately causes cell death in growing roots of Arabidopsis thaliana (L.) Heynh.
Boron deprivation immediately causes cell death in growing roots of Arabidopsis thaliana (L.) Heynh.
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DOI：
10.1080/00380768.2013.813382
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发表时间：
2013-08
影响因子：
2
通讯作者：
Yuki Oiwa;Kahori Kitayama;Masaru Kobayashi;T. Matoh
中科院分区：
文献类型：
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作者：
Yuki Oiwa;Kahori Kitayama;Masaru Kobayashi;T. Matoh
硼(B)是果胶中硼酸酯交联的一种组分，对细胞壁结构的正确形成至关重要。之前，我们发现从培养基中去除B能立即诱导悬浮培养的烟草细胞产生应激反应，但细胞表现出这种快速反应的机制尚不清楚。在本研究中，我们描述了拟南芥（Arabidopsis thaliana， L.）的早期反应。Heynh。B剥夺。剥夺B处理1 h会导致细胞死亡，特别是在根伸长区。活性氧在同一区域积聚，表明死亡是由氧化损伤引起的。B剥夺处理还能在1 h内诱导应激反应基因的表达。这些结果表明，拟南芥能立即感知并响应外界培养基中B的去除。钙的缺乏也会引起类似的反应，而镁或钾的缺乏则不会，这表明生长细胞中果胶分子交联失败触发了这些快速反应。
Abstract Boron (B) is essential for the correct formation of cell wall structure because it is a component of borate-ester cross-links in pectin. Previously, we showed that removal of B from the culture medium immediately induced stress responses in suspension-cultured tobacco (Nicotiana tabacum L.) cells, but the mechanism by which cells exhibit such rapid responses remained unclear. In this study, we characterized the early responses of Arabidopsis thaliana (L.) Heynh. to B deprivation. Deprivation of B for 1 h caused cell death specifically in the root elongation zone. Reactive oxygen species accumulated in the same region, suggesting that the death was caused by oxidative damage. The B-deprivation treatment also induced the expressions of stress-responsive genes within 1 h. These results demonstrated that A. thaliana immediately senses and responds to the removal of B from the external medium. Similar responses were induced by calcium deprivation but not magnesium or potassium deprivation, suggesting that the failure of cross-linking in pectin molecules in growing cells triggers these rapid responses.