Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish
Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish
复制标题
Dcp2 和 Cnot7 对斑马鱼母体 mRNA 清除的普遍但不均匀的贡献
DOI：
10.1111/gtc.12504
复制
发表时间：
2017
期刊：
影响因子：
2.1
通讯作者：
Tomari Yukihide
中科院分区：
文献类型：
--
作者：
Mishima Yuichiro;Tomari Yukihide
mRNA降解是一个基本的生物学过程，从细胞中清除转录的遗传信息。在动物发育的母体到合子的转变过程中，成千上万的母体mRNA通过多种机制降解，包括microRNA和密码子介导的衰变。然而，母体mRNA清除的酶促要求尚未完全了解。在这里，我们通过过度表达无催化活性的Dcp2并进行RNA测序分析，分析了脱帽酶Dcp2对斑马鱼母体mRNA清除的贡献。正如预期的那样，Dcp2在母体mRNA清除中具有广泛的作用。有趣的是，每种mRNA对Dcp2介导的去帽和Cnot7介导的去腺苷化降解表现出不同的依赖性。相关性分析确定了几个mRNA的功能，与观察到的差异依赖性。我们的研究结果表明，普遍但不均匀的贡献脱帽酶Dcp2和去腺苷酸酶Cnot7母体mRNA清除。
mRNA degradation is a fundamental biological process that erases transcribed genetic information from cells. During maternal‐to‐zygotic transition of animal development, thousands of maternal mRNAs are degraded by multiple mechanisms including microRNAs and codon‐mediated decay. Enzymatic requirements for maternal mRNA clearance, however, are not fully understood. Here, we analyzed a contribution of the decapping enzyme Dcp2 to maternal mRNA clearance in zebrafish by over‐expressing catalytically inactive Dcp2 and performing RNA‐seq analysis. As expected, Dcp2 had a widespread role in maternal mRNA clearance. Interestingly, each mRNA showed differential dependency on Dcp2‐mediated decapping and Cnot7‐mediated deadenylation for degradation. Correlation analysis identified several mRNA features that were associated with the observed differential dependency. Our results show pervasive yet nonuniform contributions of the decapping enzyme Dcp2 and the deadenylase Cnot7 to maternal mRNA clearance.