Downstream transport and seasonal variation in freshwater pearl mussel ( Margaritifera margaritifera ) eDNA concentration
Downstream transport and seasonal variation in freshwater pearl mussel (
Margaritifera margaritifera
) eDNA concentration
复制标题
淡水珍珠贝 (Margaritifera margaritifera) eDNA 浓度的下游运输和季节变化
DOI：
10.1002/edn3.10
复制
发表时间：
2019
影响因子：
--
通讯作者：
S. Karlsson
中科院分区：
文献类型：
--
作者：
S. Wacker;F. Fossøy;B. M. Larsen;Hege Brandsegg;R. Sivertsgård;S. Karlsson
资助信息NINA战略研究所倡议，赠款/奖号：遗传方法的开发和实施摘要环境DNA（eDNA）可用于检测水样中水生生物的存在和丰度。在实施这种方法作为监测工具之前，需要更多的知识，在eDNA浓度的变化与物种丰度和潜在的混杂因素。eDNA的脱落和衰变可能在整个季节变化很大，并取决于环境因素，如水温和生物过程，如活动水平和繁殖。在lotic系统中，eDNA浓度也受到eDNA下游转运的影响。固着贝类为研究河口系统中物种空间分布与eDNA含量的关系提供了一个理想的研究系统。我们量化了淡水珍珠贻贝（Margaritifera margaritifera）eDNA浓度在四个地方在一条天然河流中的贻贝空间分布的详细知识：（a）上游的已知物种分布，下游（B）一个小的和（c）一个大的聚集和（d）1,700米的下游的大聚集。为了研究季节性变化，我们在三个时期定量了eDNA浓度：（a）春末，冷水和相对不活跃的贻贝;（B）仲夏，水温较高，贻贝过滤活跃;（c）夏末，幼虫释放期间。物种检测是高度可靠的，没有检测到的eDNA上游的物种分布和完整的检测下游的大聚集。小聚集体的检测成功率很低，13%的样本检测呈阳性。下游运输是有效的，在1,700米的河流距离没有显着减少eDNA浓度。季节性变化很强，在繁殖期间，从春末到夏末，eDNA浓度增加了20倍。我们的研究结果突出了在lotic系统中eDNA监测的潜力和挑战。
Funding information NINA Strategic Institute Initiative, Grant/ Award Number: Development and implementation of genetic methods Abstract Environmental DNA (eDNA) can be used to detect the presence and abundance of aquatic organisms from water samples. Before implementing this methodology as a tool for monitoring, more knowledge is needed on variation in eDNA concentrations in relation to species abundance and potential confounding factors. Shedding and decay of eDNA may vary extensively over the season and are dependent on environmental factors such as water temperature and on biological processes such as activity level and reproduction. In lotic systems, eDNA concentrations are also affected by downstream transport of eDNA. Sessile freshwater mussels provide an ideal study system for investigating the relationship between species spatial distribution and eDNA concentrations in lotic systems. We quantified freshwater pearl mussel (Margaritifera margaritifera) eDNA concentrations at four localities in a natural river with detailed knowledge of mussel spatial distribution: (a) upstream of the known species distribution, just downstream (b) a small and (c) a large aggregation and (d) 1,700 m downstream of the large aggregation. To study seasonal variation, we quantified eDNA concentrations during three periods: (a) in late spring, with cold water and relatively inactive mussels; (b) in mid‐summer, with higher water temperature and active mussel filtration; and (c) in late summer, during the release of larvae. Species detection was highly reliable, with no detection of eDNA upstream of the species distribution and complete detection downstream of the large aggregation. Detection success of the small aggregation was low, with 13% of the samples testing positive. Downstream transport was efficient, with no significant decrease in eDNA concentrations over 1,700 m river distance. Seasonal variation was strong, with a 20‐fold increase in eDNA concentrations from late spring to late summer, during reproduction. Our results highlight both the potential and challenges of eDNA monitoring in lotic systems.
用于线性混合模型中偏差校正的参数引导方法。
影响因子：
1.6
作者：
Wali Ullah;Yoshihiko Tsukuda and Yasumasa Matsuda;田中秀夫;中溝和弥;櫻井公人・伊豆久・溝端佐登史;元橋一之;T. Kubokawa and B. Nagashima
通讯作者：
T. Kubokawa and B. Nagashima