INVIVO ASSESSMENT OF DRUG SENSITIVITY OF AFRICAN TRYPANOSOMES USING THE AKINETOPLASTIC INDUCTION TEST
INVIVO ASSESSMENT OF DRUG SENSITIVITY OF AFRICAN TRYPANOSOMES USING THE AKINETOPLASTIC INDUCTION TEST
复制标题
DOI：
10.1016/0034-5288(92)90017-v
复制
发表时间：
1992-03-01
影响因子：
2.4
通讯作者：
ONO, T
中科院分区：
文献类型：
--
作者：
CHITAMBO, H;ARAKAWA, A;ONO, T
用不同剂量的氯化异咪胺或醋酸二氮嗪治疗感染刚果锥虫或布氏锥虫的小鼠后，在给药 10 小时内，在这两个物种的锥鞭毛体中观察到促动体 (AK) 形式的诱导。 AK 诱导水平与使用标准小鼠体内药物测定发现的每种化合物的耐药性水平密切相关。一般来说，任何一种化合物的无效剂量都会导致 AK 诱导率低于 30%；复发病例的发生率在 30% 至 50% 之间，而治疗剂量的 AK 诱导率则为 50% 或更高。因此，使用普通光学显微镜体内测定 AK 诱导率是一种潜在可行的替代指标，可替代传统使用小鼠感染和治疗方法来评估非洲锥虫的药物敏感性。
Following treatment of mice infected with Trypanosoma congolense or T brucei brucei with various doses of isometamidium chloride or diminazene aceturate, the induction of akinetoplastic (AK) forms was observed in the trypomastigotes of both species within 10 hours of drug administration. The levels of AK-induction were closely correlated with the levels of resistance to each compound found using a standard in vivo drug assay in mice. In general, ineffective doses of either compound conferred AK-induction rates of less than 30 per cent; relapsing cases had between 30 and 50 per cent while curative doses had AK-induction rates of 50 per cent or more. In vivo determination Of AK-induction rates using ordinary light microscopy is thus a Potentially feasible alternative indicator to the conventional use of mice infection and treatment methods for assessing drug sensitivity in African trypanosomes.