PAR-4 and hTERT expression are negatively correlated after RNA interference targeting hTERT in laryngocarcinoma cells
PAR-4 and hTERT expression are negatively correlated after RNA interference targeting hTERT in laryngocarcinoma cells
复制标题
喉癌细胞中针对 hTERT 的 RNA 干扰后 PAR-4 和 hTERT 表达呈负相关
DOI：
10.1016/j.tice.2010.08.002
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发表时间：
2010-12-01
期刊：
影响因子：
2.6
通讯作者：
Tao, Ze-Zhang
中科院分区：
文献类型：
--
作者：
Sheng, Jian-Fei;Chen, Wei;Tao, Ze-Zhang
我们研究了喉癌细胞中端粒酶活性与人端粒酶逆转录酶 (hTERT) 和前列腺凋亡反应 4 (Par-4) 蛋白表达之间的关系。用针对 hTERT 的短发夹 RNA (shRNA) 处理后，hTERT 蛋白表达受到抑制，而 Par-4 表达增加。这些变化具有统计学意义（p < 0.05），Par-4 和 hTERT 表达呈负相关（p < 0.05，r = -0.908）。然而，我们发现端粒酶活性和 hTERT 表达之间没有相关性。我们的研究结果表明，hTERT 通过端粒酶独立途径发挥其抗凋亡作用，该途径可能与 Par-4 凋亡途径交叉。 (C) 2010 Elsevier Ltd. 保留所有权利。
We investigated the relationship between telomerase activity and the expression of human telomerase reverse transcriptase (hTERT) and prostate apoptosis response 4 (Par-4) proteins in laryngocarcinoma cells. Following treatment with short hairpin RNA (shRNA) against hTERT, hTERT protein expression was inhibited, while Par-4 expression was increased. These changes were statistically significant (p < 0.05), with Par-4 and hTERT expression being negatively correlated (p < 0.05, r = -0.908). However, we found no correlation between telomerase activity and hTERT expression. Our findings suggest that hTERT exerts its anti-apoptotic effects through a telomerase-independent pathway, which is likely to intersect with the Par-4 apoptosis pathway. (C) 2010 Elsevier Ltd. All rights reserved.