Attachment of Streptomyces coelicolor is mediated by amyloidal fimbriae that are anchored to the cell surface via cellulose
Attachment of Streptomyces coelicolor is mediated by amyloidal fimbriae that are anchored to the cell surface via cellulose
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DOI：
10.1111/j.1365-2958.2009.06838.x
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发表时间：
2009-09-01
影响因子：
3.6
通讯作者：
Claessen, Dennis
中科院分区：
文献类型：
--
作者：
de Jong, Wouter;Wosten, Han A. B.;Claessen, Dennis
ChpA-H蛋白使丝状细菌通过组装成具有表面活性的淀粉样原纤维，形成可繁殖的空中结构。我们在这里证明了粘滞物也介导了葡萄球菌对表面的附着。附着与毛的形成一致，毛通过尖状突起连接到细胞表面。质谱、电镜和刚果红处理表明，这些纤维是由束状的淀粉样原纤维组成的。在一个菌株中，卓别林基因chpA-H被灭活，附着和毛的形成被取消。相反，在这个突变体中，非常薄的原纤维从穗状突起中出现。这些原纤维对纤维素酶处理敏感。这种酶处理也从细胞表面释放了野生型菌毛，从而消除了附着。预测的纤维素合酶基因失活的菌株的附着减少也表明纤维素在表面附着中的作用。我们提出，通过纤维素锚定淀粉样菌毛的附着机制在细菌中广泛存在，可能在感染的开始和生物膜的形成中起作用。
P>The chaplin proteins ChpA-H enable the filamentous bacterium Streptomyces coelicolor to form reproductive aerial structures by assembling into surface-active amyloid-like fibrils. We here demonstrate that chaplins also mediate attachment of S. coelicolor to surfaces. Attachment coincides with the formation of fimbriae, which are connected to the cell surface via spike-shaped protrusions. Mass spectrometry, electron microscopy and Congo red treatment showed that these fimbriae are composed of bundled amyloid fibrils of chaplins. Attachment and fimbriae formation were abolished in a strain in which the chaplin genes chpA-H were inactivated. Instead, very thin fibrils emerged from the spike-shaped protrusions in this mutant. These fibrils were susceptible to cellulase treatment. This enzymatic treatment also released wild-type fimbriae from the cell surface, thereby abolishing attachment. The reduced attachment of a strain in which the gene of a predicted cellulose synthase was inactivated also indicates a role of cellulose in surface attachment. We propose that the mechanism of attachment via cellulose-anchored amyloidal fimbriae is widespread in bacteria and may function in initiation of infection and in formation of biofilms.