Klebsiella pneumoniae type 3 pili facilitate adherence and biofilm formation on abiotic surfaces
Klebsiella pneumoniae type 3 pili facilitate adherence and biofilm formation on abiotic surfaces
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DOI：
10.1016/s0923-2508(02)00004-9
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发表时间：
2003-01-01
影响因子：
2.6
通讯作者：
Darfeuille-Michaud, A
中科院分区：
文献类型：
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作者：
Di Martino, P;Cafferini, N;Darfeuille-Michaud, A
通过对26株临床菌株、7株配水系统菌株和4株参考菌株的分析，评估了I型和3型皮利在介导惰性表面肺炎克雷伯菌定植中的作用。在测试的33个野生型菌株中，57.6%表达1型皮利，54.5%表达3型皮利，42.4%是高度疏水性的，69.7%是高度粘附于玻璃和聚丙烯表面的，48.5%是在聚苯乙烯培养皿上有效的生物膜形成菌株。高粘附性和生物膜形成与细菌表面疏水性、3型菌毛表达呈正相关，但与I型皮利表达无关，并且不依赖于菌株的来源。酸克雷仅表达I型菌毛的肺炎CF3051参考菌株轻微粘附于玻璃和聚丙烯，并且在聚苯乙烯上不形成生物膜。K.产生I型和3型菌毛的肺炎IA565和CF3097参考菌株显示出对玻璃和聚丙烯的有效粘附以及在聚苯乙烯上的生物膜形成。此外，用携带mrk基因簇的重组pFK10质粒转化无菌毛的弱粘附性CF 3172菌株导致3型菌毛表达、表面疏水性增加、对非生物表面的粘附性增加和生物膜形成。因此，3型皮利构成主要的K。pneumoniae粘附因子，促进不同来源的菌株在非生物表面上的粘附和生物膜形成。(C)2002年，Elsevier SAS科学与医学版。All rights reserved.
The role of type I and type 3 pili in mediating Klebsiella pneumoniae colonization of inert surfaces was assessed by analysis of 26 clinical, 7 water distribution system and four reference strains. Amongst the 33 wild type strains tested, 57.6% expressed type 1 pili, 54.5% expressed type 3 pili, 42.4% were highly hydrophobic, 69.7% were highly adherent to both glass and polypropylene surfaces, and 48.5% were efficient biofilm-forming strains on polystyrene dishes. High adherence and biofilm formation were positively correlated with bacterial surface hydrophobicity, type 3 fimbriae expression but not with type I pili expression and were not dependent upon the strain's origin. The K. pneumoniae CF3051 reference strain expressing only type I fimbriae adhered slightly to glass and polypropylene and did not form biofilm on polystyrene. K. pneumoniae IA565 and CF3097 reference strains producing type I and type 3 fimbriae showed efficient adherence to both glass and polypropylene and biofilm formation on polystyrene. Moreover, transformation of the afimbriated, weakly-adherent CF3172 strain with the recombinant pFK10 plasmid carrying the mrk gene cluster resulted in type 3 fimbriae expression, increased surface hydrophobicity, increased adherence to abiotic surfaces and biofilm formation. Thus, type 3 pili constitute the main K. pneumoniae adhesive factor, facilitating adherence and biofilm formation on abiotic surfaces of strains of different origins. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.