A family of candidate taste receptors in human and mouse
A family of candidate taste receptors in human and mouse
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DOI：
10.1038/35007072
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发表时间：
2000-04-06
期刊：
影响因子：
64.8
通讯作者：
Buck, LB
中科院分区：
文献类型：
--
作者：
Matsunami, H;Montmayeur, JP;Buck, LB
哺乳动物的味觉系统可以感觉到四种基本的味道，苦、甜、咸、酸，以及鲜味，谷氨酸的味道(1-6)。以前的研究表明，口腔中味觉感受器细胞对苦味和甜味的检测可能涉及G蛋白偶联受体(2，7，8)。虽然已经确定了两种推测的G蛋白偶联的苦味/甜味受体(9)，但苦味和甜味化合物的化学多样性使人们预计会有更多不同的受体(8，10，11)。在这里，我们报告了一个候选味觉受体家族(TRBs)的鉴定，它们是G蛋白偶联受体超家族的成员，由味觉受体细胞特异性表达。编码人类TRBs的一组基因位于PRP基因座位(12)附近，在小鼠中，PRP基因座位与参与检测苦味化合物蔗糖八乙酸酯(13-15)的SOA遗传座位紧密相连。另一个TRB基因在人类5p15染色体的重叠群上被发现，5p15是控制人类苦味化合物6-正丙基-2-硫氧尿嘧啶检测的遗传位点(PROP)的位置(16，17)。
The gustatory system of mammals can sense four basic taste qualities, bitter, sweet, salty and sour, as well as umami, the taste of glutamate(1-6). Previous studies suggested that the detection of bitter and sweet tastants by taste receptor cells in the mouth is likely to involve G-protein-coupled receptors(2,7,8). Although two putative G-protein-coupled bitter/sweet taste receptors have been identified(9), the chemical diversity of bitter and sweet compounds leads one to expect that there is a larger number of different receptors(8,10,11). Here we report the identification of a family of candidate taste receptors (the TRBs) that are members of the G-protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells. A cluster of genes encoding human TRBs is located adjacent to a Prp gene locus(12), which in mouse is tightly linked to the SOA genetic locus that is involved in detecting the bitter compound sucrose octaacetate(13-15). Another TRB gene is found on a human contig assigned to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil in humans(16,17).