Surface interactions between Escherichia coli and hemocytes of the Mediterranean mussel Mytilus galloprovincialis Lam. leading to efficient bacterial clearance
Surface interactions between Escherichia coli and hemocytes of the Mediterranean mussel Mytilus galloprovincialis Lam. leading to efficient bacterial clearance
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DOI：
10.1128/aem.67.1.464-468.2001
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发表时间：
2001-01-01
影响因子：
4.4
通讯作者：
Gallo, G
中科院分区：
文献类型：
--
作者：
Canesi, L;Pruzzo, C;Gallo, G
1 型菌毛在大肠杆菌和贻贝相互作用中的作用。评估血细胞。 18℃下，在人工海水和血淋巴血清中，有伞毛的菌株MG155与单层血细胞的关联分别是无伞毛突变体AAEC072的1.5倍和3至4倍。这种差异显然是由于不同的粘附特性造成的，因为当血细胞在 4°C 下孵育以抑制内化过程时，MG155 比 AAEC072 粘附更有效。血淋巴血清使 MG155 的结合和粘附增加两到三倍，但不影响 AAEC072 的结合和粘附。根据孵育 60 和 120 分钟后可培养细菌的数量评估，MG155 对血细胞杀伤的敏感性比 AAEC072 高 1.5 至 1.7 倍。 D-甘露糖的抑制作用证实了 1 型菌毛在 MG155 与血细胞相互作用中的作用。在体内实验中，MG155 细胞从循环血淋巴中的清除速度比 AAEC072 细胞的清除速度更快。这些结果证实，表面特性对于影响贻贝血淋巴内细菌的持久性和存活至关重要。
The role of type 1 fimbriae in the interactions between Escherichia coli and Mytilus galloprovincialis Lam. hemocytes was evaluated. The association of fimbriated strain MG155 with hemocyte monolayers at 18 degreesC was 1.5- and 3- to 4-fold greater than the association of unfimbriated mutant AAEC072 in artificial seawater and in hemolymph serum, respectively. Such differences were apparently due to different adhesive properties since MG155 adhered more efficiently than AAEC072 when hemocytes were incubated at 4 degreesC to inhibit the internalization process. Hemolymph serum increased both association and adherence of MG155 two- to threefold but did not affect association and adherence of AAEC072. MG155 was also 1.5 to 1.7-fold more sensitive to killing by hemocytes than AAEC072, as evaluated by the number of culturable bacteria after 60 and 120 min of incubation. The role of type 1 fimbriae in MG155 interactions with hemocytes was confirmed by the inhibitory effect of D-mannose. In in vivo experiments MG155 cells were cleared from circulating hemolymph more rapidly than AAEC072 cells were cleared. These results confirm that surface properties are crucial in influencing bacterial persistence and survival within mussel hemolymph.