Characterization of an African trypanosome mutant refractory to lectin-induced death.
Characterization of an African trypanosome mutant refractory to lectin-induced death.
复制标题
对凝集素诱导的死亡具有抵抗力的非洲锥虫突变体的表征。
DOI：
10.1016/j.bbrep.2015.08.013
复制
发表时间：
2015
影响因子：
2.7
通讯作者：
Morris,JamesC
中科院分区：
文献类型：
--
作者：
Hackler,AmberL;Qiu,Yijian;Patrick,StephenL;Lee,SooHee;Acosta-Serrano,Alvaro;Morris,JamesC
Incubation of African trypanosomes with the lectin concanavalin A (conA) leads to alteration in cellular DNA content, DNA degradation, and surface membrane blebbing. Here, we report the generation and characterization of a conA-refractoryTrypanosoma bruceiline. These insect stage parasites were resistant to conA killing, with a median lethal dose at least 50-fold greater than the parental line. Fluorescence-based experiments revealed that the resistant cells bound less lectin when compared to the parental line. Western blotting and mass spectrometry confirmed that the resistant line lacked an N-glycan required for conA binding on the cellular receptors, EP procyclin proteins. The failure to N-glycosylate the EP procyclins was not the consequence of altered N-glycan precursor biosynthesis, as another glycosylated protein (Fla1p) was normally modified. These findings support the likelihood that resistance to conA was a consequence of failure to bind the lectin trigger.
