Molecular characterization of phospholipase c (AaPLC) in infection structures differentiation induced by pear fruit surface signals, stress responses, secondary metabolism and virulence of Alternaria alternata
Molecular characterization of phospholipase c (AaPLC) in infection structures differentiation induced by pear fruit surface signals, stress responses, secondary metabolism and virulence of Alternaria alternata
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梨果实表面信号、应激反应、次生代谢和链格孢毒力诱导的感染结构分化中磷脂酶 c (AaPLC) 的分子特征
DOI：
10.1094/phyto-11-21-0475-r
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Prusky Dov B.
中科院分区：
文献类型：
--
作者：
Huang Yi;Li Yongcai;Li Dongmei;Bi Yang;Liu Yongxiang;Mao Renyan;Zhang Miao;Jiang Qianqian;Wang Xiaojing;Prusky Dov B.
真菌病原体利用植物表面的物理化学信号来触发特定的发育过程。为了评估磷脂酶C （PLC）在互花莲子介导植物刺激感知中的作用，通过构建ΔAaPLC突变体对三个PLC基因的功能进行了表征。结果表明，果蜡包覆表面显著诱导了异交蜜及其突变体附着胞的形成（P0.05）。ΔAaPLC突变体的萌发与野生型没有差异。aplc1的缺失导致附着胞和感染菌丝减少，但仍能识别不同蜡的刺激，其中对梨蜡的反应最强。在以梨蜡（θ4）为载体的脱蜡洋葱表皮上，ΔAaPLC1突变体培养8 h后附着菌丝的形成和侵染菌丝的形成分别减少了14.5%和65.7% (P0.05)，而ΔAaPLC2和ΔAaPLC3的侵染菌丝的形成与野生型相同（P0.05）。此外，aplc1突变对真菌的生物学功能产生了多效性影响，包括生长缺陷、胁迫耐受性改变、对宿主致病性减弱、真菌毒素合成破坏等。发现AaPLC2和AaPLC3基因对胁迫反应和霉菌毒素产生有一定的影响。综上所述，AaPLC基因对褐刺草的生长、应激反应、致病性和次生代谢有差异调控。
Fungal pathogens use plant surface physiochemical signals to trigger specific developmental processes. To assess the role of Phospholipase C (PLC) in mediating plant stimuli sensing of Alternaria alternata, function of three PLC genes were characterized by constructing ΔAaPLC mutants. Here we showed that the fruit wax-coated surfaces significantly induced appressorium formation in A. alternata and mutants (P0.05). Germination of ΔAaPLC mutants did not differ from the wild-type. Deletion of AaPLC1 led to a reduction in appressorium and infected hyphae, but it was still recognized the stimulation from different waxes, with the strongest response to pear wax. Appressorium formation and infected hyphae of the ΔAaPLC1 mutant on dewaxed onion epidermis mounted with pear wax (θ4) were reduced by 14.5 % and 65.7% after 8 h incubation (P0.05), while the ΔAaPLC2 and ΔAaPLC3 formed the same infection hyphae as the wild-type (P0.05). In addition, AaPLC1 mutation caused pleiotropic effects on fungal biological function, including growth deficiency, changes in stress tolerance, weakening of pathogenicity to the host, as well as destruction of mycotoxin synthesis. Both the AaPLC2 and AaPLC3 genes were found to have some effects on stress response and mycotoxin production. Taken together, AaPLC genes differentially regulate the growth, stress response, pathogenicity and secondary metabolism of A. alternata.