Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat
Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat
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DOI：
10.1016/j.ijfoodmicro.2011.04.028
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发表时间：
2011-07-15
影响因子：
5.4
通讯作者：
Jaoua, Samir
中科院分区：
文献类型：
--
作者：
Ben Hsouna, Anis;Trigui, Mohamed;Jaoua, Samir
本研究描述了植物化学概况和保护作用的角豆豆荚精油（CsEO），食品和药用植物广泛分布在突尼斯。在CsEO中识别出25种不同的组分。其中主要检出的成分有：十九烷、二十一烷、萘、1，2-苯二甲酸二丁酯、十七烷、十六烷酸、十八烷酸、1，2-苯二甲酸、惕各酸苯乙酯、二十烷醇、金合欢醇3、樟脑、橙花叔醇和正二十烷。采用琼脂扩散法和肉汤微量稀释法评价了CsEO对13种细菌和8种真菌菌株的抗菌活性。结果表明，CsEO对受试种属表现出中等至较强的抗菌活性。此外，在体内评价了这种CsEO对食源性病原体单核细胞增生李斯特菌的抑制作用，实验性地接种在用不同浓度的CsEO修正的碎牛肉（2 × 10（2）CFU/g肉）中，并在7 ℃下储存10天。CsEO在碎牛肉中的抗菌活性非常明显，其存在导致在7 ℃下对病原体的强烈抑制作用。另一方面，通过MTT法检测挥发油对两种肿瘤细胞系HeLa和MCF-7的细胞毒作用。CsEO对两种细胞系均有抑制作用，对HeLa细胞的活性明显增强。对HeLa和MCF-7细胞的IC 50值分别为210和800 μ g/ml。总体而言，这里提出的结果表明，环氧乙烷的C。长角果具有抗微生物和细胞毒性特性，因此是食品和制药工业活性成分的潜在来源。(C)2011爱思唯尔有限公司版权所有。
The present study describes the phytochemical profile and the protective effects of Ceratonia siliqua pods essential oil (CsEO), a food and medicinal plant widely distributed in Tunisia. Twenty five different components were identified in the CsEO. Among them, the major detected components were: Nonadecane, Heneicosane, Naphthalene, 1,2-Benzenedicarboxylic acid dibutylester, Heptadecane, Hexadecanoic acid, Octadecanoic acid, 1,2-Benzenedicarboxylic acid, Phenyl ethyl tiglate, Eicosene, Farnesol 3, Camphor, Nerolidol and n-Eicosane. The antimicrobial activity of CsEO was evaluated against a panel of 13 bacteria and 8 fungal strains using agar diffusion and broth microdilution methods. Results have shown that CsEO exhibited moderate to strong antimicrobial activity against the tested species. In addition, the inhibitory effect of this CsEO was evaluated in vivo against a foodborne pathogens Listeria monocytogenes, experimentally inoculated in minced beef meat (2 x 10(2) CFU/g of meat) amended with different concentrations of the CsEO and stored at 7 degrees C for 10 days. The antibacterial activity of CsEO in minced beef meat was clearly evident and its presence led to a strong inhibitory effect against the pathogens at 7 degrees C. On the other hand, the cytotoxic effects of the essential oil against two tumoral human cell lines HeLa and MCF-7 were examined by MTT assay. The CsEO showed an inhibition of both cell lines with significantly stronger activity against HeLa cells. The IC50 values were 210 and 800 mu g/ml for HeLa and MCF-7 cells, respectively. Overall, results presented here suggest that the EO of C. siliqua possesses antimicrobial and cytotoxic properties, and is therefore a potential source of active ingredients for food and pharmaceutical industry. (C) 2011 Elsevier B.V. All rights reserved.