A glucose biosensor based on chitosan-glucose oxidase-gold nanoparticles biocomposite formed by one-step electrodeposition
A glucose biosensor based on chitosan-glucose oxidase-gold nanoparticles biocomposite formed by one-step electrodeposition
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DOI：
10.1016/j.ab.2004.07.005
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发表时间：
2004-11-15
影响因子：
2.9
通讯作者：
Chen, HY
中科院分区：
文献类型：
--
作者：
Luo, XL;Xu, JJ;Chen, HY
报道了一种用于葡萄糖定量测量的电流型生物传感器。该生物传感器是基于一种均匀且易于制备的生物复合材料。这种生物复合材料是由壳聚糖水凝胶、葡萄糖氧化酶和金纳米颗粒在酶友好的条件下通过直接、简便的电化学沉积方法制成的。由此得到的生物复合材料为酶在相当极端的条件下保持其生物活性提供了保护层，生物复合材料中修饰的金纳米颗粒对酶具有良好的亲和力。该传感器对葡萄糖的检测具有较快的响应速度(在7 S范围内)，线性范围为5.0~2.4 mm，检出限为2.7um。将金纳米颗粒的亲和力和生物复合材料的前景与一步法非人工技术相结合，有利于提高分析能力，从而有助于灵敏地测定葡萄糖。(C)2004 Elsevier Inc.保留所有权利。
An amperometric biosensor for the quantitative measurement of glucose is reported. The biosensor is based on a biocomposite that is homogeneous and easily prepared. This biocomposite is made of chitosan hydrogel, glucose oxidase, and gold nanoparticles by a direct and facile electrochemical deposition method under enzyme-friendly conditions. The resulting biocomposite provided a shelter for the enzyme to retain its bioactivity at considerably extreme conditions, and the decorated gold nanoparticles in the biocomposite offer excellent affinity to enzyme. The biosensor exhibited a rapid response (within 7 s) and a linear calibration range from 5.0 muM to 2.4 mM with a detection limit of 2.7 muM for the detection of glucose. The combination of gold nanoparticles affinity and the promising feature of the biocomposite with the onestep nonmanual technique favor the sensitive determination of glucose with improved analytical capabilities. (C) 2004 Elsevier Inc. All rights reserved.