Possible involvement of the peripheral Mu-opioid system in antinociception induced by bergamot essential oil to allodynia after peripheral nerve injury
Possible involvement of the peripheral Mu-opioid system in antinociception induced by bergamot essential oil to allodynia after peripheral nerve injury
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DOI：
10.1016/j.neulet.2018.08.053
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发表时间：
2018-11-01
影响因子：
2.5
通讯作者：
Sakurada, Tsukasa
中科院分区：
文献类型：
--
作者：
Komatsu, Takaaki;Katsuyama, Soh;Sakurada, Tsukasa
佛手柑精油（BEO）是最常见的精油之一，也是大众最熟悉的。本研究的目的是调查足底内（i.pl.）BEO对部分坐骨神经结扎（PSNL）诱发的小鼠神经病理性异常性疼痛的影响及其阿片受体亚型的研究我们的研究结果表明，单剂量的i.pl.使用vonFrey试验，BEO的施用显著抑制PSNL诱导的神经性疼痛。在von Frey试验中，用纳洛酮甲碘化物（一种外周作用μ阿片受体偏好拮抗剂）、β-盐酸funaltamine（β-FNA）（一种选择性β-阿片受体拮抗剂）和β-内啡肽抗血清进行预处理可显著逆转BEO的抗异常性疼痛作用，但naltrindole（非选择性δ-阿片受体拮抗剂）和nor-binaltorphimine（选择性κ-阿片受体拮抗剂）不能逆转BEO的抗异常性疼痛作用。i.pl此外，在蛋白质印迹分析中，i.pl. BEO的给药导致PSNL诱导的脊髓细胞外信号调节蛋白激酶（ERIC）活化的显著阻断。纳洛酮甲碘化物和β-FNA显着逆转了BEO诱导的脊髓ERIC激活的阻滞。这些结果表明，i.pl.注射BEO诱导的抗异常性疼痛作用和脊髓ERK激活的阻断可以通过外周μ-阿片受体的激活来触发。
The essential oil of bergamot (BEO) is one of the most common essential oils and is most familiar to the general public. The aims of this study were to investigate the effect of intraplantar (i.pl.) BEO on neuropathic allodynia induced by partial sciatic nerve ligation (PSNL) in mice and the opioid receptor subtypes involved in the antiallodynic effects of BEO. Our findings showed that a single dose of i.pl. administration of BEO significantly inhibited the PSNL-induced neuropathic pain using the von Frey test. The i.pl pretreatment with naloxone methiodide, a peripherally acting mu-opioid receptor preferring antagonist, beta-funaltrexamine hydrochloride (beta-FNA), a selective p-opioid receptor antagonist, and beta-endorphin antiserum significantly reversed the antiallodynic effect of BEO in the von Frey test, but not by naltrindole, the nonselective delta-opioid receptor antagonist and nor-binaltorphimine, the selective kappa-opioid receptor antagonist. Furthermore, in the western blotting analysis, i.pl. administration of BEO resulted in a significant blockage of spinal extracellular signal-regulated protein kinase (ERIC) activation induced by PSNL. Naloxone methiodide and beta-FNA significantly reversed the blockage of spinal ERIC activation induced by BEO. These results suggest that i.pl. injection of BEO-induced antiallodynic effect and blockage of spinal ERK activation may be triggered by activation of peripheral mu-opioid receptors.