Submucosal Fibrosis and Basic-Fibroblast Growth Factor-Positive Neutrophils Correlate with Colonic Stenosis in Cases of Ulcerative Colitis
Submucosal Fibrosis and Basic-Fibroblast Growth Factor-Positive Neutrophils Correlate with Colonic Stenosis in Cases of Ulcerative Colitis
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DOI：
10.1159/000320773
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发表时间：
2011-01-01
期刊：
影响因子：
3.2
通讯作者：
Okayasu, Isao
中科院分区：
文献类型：
--
作者：
Yamagata, Masaru;Mikami, Tetuo;Okayasu, Isao
背景和目的：溃疡性结肠炎(UC)的良性狭窄发生率较低，报道为3.2-11.2%，粘膜下层或深层纤维化是其原因之一。本研究的目的是用免疫组织化学染色方法评估UC患者的狭窄程度，并分析狭窄和非狭窄患者之间的差异，重点是碱性成纤维细胞生长因子(b-FGF)的表达和肌成纤维细胞。方法：对9例狭窄UC和17例非狭窄UC进行组织病理学检查和免疫组织化学染色，检测b-FGF、α-SMA、CD34、CD68和IL-6的表达。采用b-FGF和髓过氧化物酶或CD68免疫组织化学双重染色方法鉴定b-FGF阳性细胞。结果：除粘膜下纤维化外，狭窄处b-FGF炎性细胞和肌成纤维细胞明显增多。大多数b-FGF阳性细胞也呈髓过氧化物酶阳性，且b-FGF阳性细胞数与中性粒细胞总数呈正相关。结论：慢性溃疡性结肠炎狭窄的主要原因之一是肠壁纤维化，可能是由炎性中性粒细胞渗入产生b-FGF所致。版权所有(C)2011 S.Karger AG，巴塞尔
Background and Aims: The frequency of benign stenosis in ulcerative colitis (UC) is low, reported as being 3.2-11.2%, with fibrosis in the submucosa or deeper pointed out as one of the causes. The aim of the present study was to assess stenosis in UC cases using immunostaining and to analyze differences between stenotic and nonstenotic cases, focusing on basic-fibroblast growth factor (b-FGF) expression and myofibroblasts. Methods: Totals of 9 stenotic and 17 nonstenotic UC cases were histopathologically examined and immunohistochemically stained for b-FGF, alpha-smooth muscle actin (alpha-SMA), CD34, CD68 and IL-6. To identify b-FGF-positive cells, double immunostaining for b-FGF and myeloperoxidase or CD68 was performed. Results: In addition to submucosal fibrosis, a significant increase of b-FGF-positive inflammatory cells and myofibroblasts was observed in stenotic portions. Most b-FGF-positive cells were also positive for myeloperoxidase, and a correlation between b-FGF-positive and total neutrophil counts was found. Conclusions: One of the major causes of stenosis in long-standing UC is fibrosis in the bowel wall, possibly induced by infiltrating inflammatory neutrophils producing b-FGF. Copyright (C) 2011 S. Karger AG, Basel