Ascorbic acid concentration in rabbit vitreous measured by microdialysis with HPLC-electrochemical detection before and after vitreous surgery
Ascorbic acid concentration in rabbit vitreous measured by microdialysis with HPLC-electrochemical detection before and after vitreous surgery
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DOI：
10.1016/j.exer.2005.10.022
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发表时间：
2006-05-01
影响因子：
3.4
通讯作者：
Hiramitsu, Tadahisa
中科院分区：
文献类型：
--
作者：
Koide, Kenro;Zhang, Xiao Mei;Hiramitsu, Tadahisa
采用高效液相色谱-电化学检测法测定玻璃体切割手术前后兔玻璃体中抗坏血酸(AA)的浓度。在玻璃体液中植入纤维素微透析探头，稳定后，在10天内每天进行AA测量。观察玻璃体切割术切除玻璃体三分之二的效果。观察曲安奈德(TA)对4组兔的作用：第1组，单纯使用TA 20 mg，第2组，单纯玻璃体内注射TA 4 mg，第3组，玻璃体切除后注射TA 20 mg，第4组，玻璃体切除后注射TA 4 mg。结果表明，玻璃体切割术后第2~10天AA浓度显著降低，第7天最大降幅为49.5%(P<0.005)。玻璃体切割术后第1、2组AA含量无明显变化，第3组AA含量轻度恢复，第4组AA含量恢复最快。TA治疗对玻璃体切割后AA含量降低有明显的抑制作用。TA的这种减弱作用可能是由于其抗炎作用阻止了血-房水屏障的破坏。(C)2005爱思唯尔有限公司。保留所有权利。
Microdialysis with high performance liquid chromatography and electrochemical detection (HPLC-ECD) was used to measure ascorbic acid (AA) concentrations in rabbit vitreous before and after vitrectomy. A cellulose microdialysis probe was implanted in the vitreous humor, and after stabilization, AA measurements were made daily over a 10 day period. The effect of removing two-thirds of the vitreous by vitrectomy was examined. The effect of triamcinolone acetonide (TA) was evaluated in four groups of rabbits: Group 1, sub-tenon TA (20 mg) alone; Group 2, intravitreous TA (4 mg) alone; Group 3, sub-tenon TA (20 mg) after vitrectomy, and Group 4, intravitreous TA (4 mg) after vitrectomy. The results showed that the AA concentration after vitrectomy was significantly lower from days 2 to10 with a maximum reduction of 49.5% (P < 0.005) on day 7. No significant changes in the AA level was observed in Groups I and 2, a mild recovery of AA concentration reduction after vitrectomy was detected in Group 3. The highest recovery of the AA concentration reduction was observed in Group 4. The attenuating effect of TA treatment on the reduction of AA in the vitreous after vitrectomy was significant. This attenuating effect of the TA may be due to prevention of the disruption of the blood-aqueous barrier by its anti-inflammatory action. (c) 2005 Elsevier Ltd. All rights reserved.