Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells
Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells
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DOI：
10.1016/j.scr.2009.11.002
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发表时间：
2010-03-01
影响因子：
1.2
通讯作者：
Elefanty, Andrew G.
中科院分区：
文献类型：
--
作者：
Heraud, Philip;Ng, Elizabeth S.;Elefanty, Andrew G.
人类ESC (hESC) 是研究人类早期发育的宝贵工具，是制药和生物技术应用以及最终用于细胞替代疗法的正常分化细胞的来源。对于所有应用，有必要开发检测方法来验证 hESC 分化的功效。我们探索了 FTIR 光谱技术（一种快速表征细胞大分子组成的技术）的能力，以区分中内胚层或外胚层定型细胞与未分化的 hESC。独特的红外光谱“特征”很容易将 hESC 与这些早期分化的后代区分开来，生物信息学模型能够正确分类超过 97% 的光谱。这些数据确定了 FTIR 光谱作为补充 hESC 及其衍生物传统分析的新模式的作用。 FTIR 光谱有潜力为工业和再生医学中使用的干细胞和分化细胞的质量控制提供低成本、自动化的测量。皇冠版权所有 (C) 2009 由 Elsevier B.V. 出版。保留所有权利。
Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic "signatures" readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to correctly classify over 97% of spectra. These data identify a role for FTIR spectroscopy as a new modality to complement conventional analyses of hESCs and their derivatives. FTIR spectroscopy has the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells to be used in industry and regenerative medicine. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.