Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro
Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro
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DOI：
10.1080/01635581.2016.1154577
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发表时间：
2016-01-01
影响因子：
2.9
通讯作者：
Przygodzki, Ronald M.
中科院分区：
文献类型：
--
作者：
Kubatka, Peter;Kello, Martin;Przygodzki, Ronald M.
本文评价了饲喂含有多种植物化学物质混合物的青年大麦对N-甲基-N-亚硝脲诱发的雌性大鼠乳腺癌的预防作用。给予致癌剂后(14wk)，取出乳腺肿瘤，进行组织病理学和免疫组织化学分析。此外，还对MCF-7乳腺癌细胞株可能的作用机制进行了体外评价。大麦(0.3%)对乳腺癌有轻微的抗癌作用，而3%大麦不能进一步提高这一作用。免疫组织化学分析显示，治疗组大鼠肿瘤细胞caspase-3表达明显增加，Ki67表达显著降低。此外，与对照相比，3%大麦显著降低了二氢酪氨酸水平。高剂量大麦能显著降低大鼠血清低密度脂蛋白-胆固醇水平。体外研究表明，与对照组相比，大麦显著降低3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium溴化实验中MCF-7细胞的存活率，并显著降低5-溴-20-脱氧尿苷的掺入。大麦抑制了细胞周期进程，与大麦延长孵育时间后，膜联蛋白V/碘化丙啶阳性的MCF-7细胞比例显著增加。我们的结果表明，在乳腺癌模型中，年轻大麦中存在的植物化学物质的混合物具有抗肿瘤作用。
The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.