The role of dynamin 3 in the testis
The role of dynamin 3 in the testis
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DOI：
10.1002/jcp.20855
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发表时间：
2007-03-01
影响因子：
5.6
通讯作者：
Vogl, A. W.
中科院分区：
文献类型：
--
作者：
Vaid, K. S.;Guttman, J. A.;Vogl, A. W.
我们在这里报告说，在睾丸中的发动蛋白3与结构称为tubulobulbar复合物，内化完整的细胞间连接在精子释放和周转的血睾屏障。该蛋白位于邻近肌动蛋白Arp 2/3网络，该网络在每个复合物的核心处将双质膜管状内陷套在一起。为了探索发动蛋白3和基于nectin的粘附连接之间的可能关系，我们将DsRed标记的发动蛋白3瞬时转染到用eGFP标记的nectin 2稳定转染的MDCK细胞中，所述nectin 2是已知在粘附连接处的支持细胞中表达的粘附分子之一。与仅表达连接蛋白2或仅用DsRed质粒转染的对照细胞相比，用发动蛋白3构建体转染的细胞在细胞间接触处具有较不均匀分布的连接蛋白2。值得注意的是，可见nectin 2阳性的管状延伸物从细胞间接触区域投射到细胞中。我们的研究结果是一致的结论，发动蛋白3参与管小叶形态发生。发动蛋白3也出现在精子尾部连接段的头状突和横纹柱周围的集中沉积物中，这表明这些细胞中的蛋白质可能起稳定尾部基部的作用，或者在受精期间或之后用作储存器。
We report here that dynamin 3 in the testis is associated with structures termed tubulobulbar complexes that internalize intact intercellular junctions during sperm release and turnover of the blood-testis barrier. The protein lies adjacent to an actin-Arp2/3 network that cuffs the double plasma membrane tubular invagination at the core of each complex. To explore the possible relationship between dynamin 3 and nectin-based adhesion junctions, we transiently transfected DsRed-tagged dynamin 3 into MDCK cells stably transfected with eGFP-tagged nectin 2, one of the adhesion molecules known to be expressed in Sertoli cells at adhesion junctions. Cells transfected with the dynamin 3 construct had less uniformly distributed nectin 2 at intercellular contacts when compared to control cells expressing only nectin 2 or transfected with the DsRed plasmid alone. Significantly, tubular extensions positive for nectin 2 were visible projecting into the cells from regions of intercellular contact. Our findings are consistent with the conclusion that dynamin 3 is involved with tubulobulbar morphogenesis. Dynamin 3 also occurs in concentrated deposits around the capitulum and striated columns in the connecting piece of sperm tails suggesting that the protein in these cells may function to stabilize the base of the tail or serve as a reservoir for use during or after fertilization.