Vessel patterning in the embryo of the zebrafish: Guidance by notochord
Vessel patterning in the embryo of the zebrafish: Guidance by notochord
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DOI：
10.1006/dbio.1996.8495
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发表时间：
1997-03-01
影响因子：
2.7
通讯作者：
Fishman, MC
中科院分区：
文献类型：
--
作者：
Fouquet, B;Weinstein, BM;Fishman, MC
我们已经克隆了受体酪氨酸激酶flk-1的斑马鱼同源物，为我们提供了一个工具来研究正常的血管模式的形成在发育中的斑马鱼胚胎，并比较它的突变体中，血管模式受到干扰。我们发现，在正常发育过程中，第一个成血管细胞出现在中胚层横向，然后迁移到内侧，形成大的轴向血管，背主动脉（轴动脉）和轴静脉的原基。管腔形成发生在受精后24小时循环开始前不久。我们研究了在突变钟形，不能形成血管和血液的血管祖细胞的规格。Cloche缺乏所有表达Flk的细胞，因此似乎缺乏成血管细胞。躯干的轴向血管形成在靠近脊索和内胚层的位置，这可能为它们的形成提供线索。背主动脉通常位于脊索的腹侧;轴静脉位于背主动脉的正下方和内胚层的正上方。浮头（flh）和无尾（ntl）突变体都在脊索的形成中具有缺陷。两者都是细胞自主性病变，flh破坏脊索，ntl阻止其分化。在这两种突变体中，背主动脉不能形成，而轴静脉的形成受到的影响较小。突变胚胎的嵌合体分析表明，移植的野生型细胞可以成为突变flh胚胎的脊索。在这些嵌合体胚胎中，表达flk的flh细胞聚集在中线，野生型脊索下方，形成主动脉原基。这表明来自脊索的信号可以引导成血管细胞形成背主动脉。脊索似乎对静脉的形成不太重要。(C)北京：科学出版社.
We have cloned the zebrafish homolog of the receptor tyrosine kinase flk-1 to provide us with a tool to study normal vascular pattern formation in the developing zebrafish embryo and to compare it to mutants in which vascular pattern is perturbed. We find that during normal development the first angioblasts arise laterally in the mesoderm and then migrate medially to form the primordia of the large axial vessels, the dorsal aorta (axial artery) and the axial vein. Lumen formation occurs shortly before onset of circulation at 24 hr postfertilization. We examined the specification of vascular progenitors in the mutant cloche, which fails to form both vessels and blood. cloche lacks all flk-expressing cells and therefore appears to lack angioblasts. The axial vessels of the trunk form in close proximity to notochord and endoderm, which may provide cues for their formation. The dorsal aorta is normally just ventral to the notochord; the axial vein is just below the dorsal aorta and above the endoderm. floating head (flh) and no tail (ntl) mutants both have defects in the formation of notochord. Both are cell-autonomous lesions, flh abolishing notochord and ntl preventing its differentiation. In both mutants the dorsal aorta fails to form, while formation of the axial vein is less affected. Mosaic analysis of mutant embryos shows that transplanted wild-type cells can become notochord in mutant flh embryos. In these mosaic embryos flh cells expressing flk assemble at the midline, beneath the wild-type notochord, and form an aortic primordium. This suggests that signals from the notochord may guide angioblasts in the fashiong of the dorsal aorta. The notochord seems to be less important for the formation of the vein. (C) 1997 Academic Press.