THE PREPARATION OF PURIFIED BLUETONGUE VIRUS GROUP ANTIGEN FOR USE AS A DIAGNOSTIC REAGENT
THE PREPARATION OF PURIFIED BLUETONGUE VIRUS GROUP ANTIGEN FOR USE AS A DIAGNOSTIC REAGENT
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DOI：
10.1007/bf01314453
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
NEWMAN, JFE
中科院分区：
文献类型：
--
作者：
GUMM, ID;NEWMAN, JFE
从蓝舌病毒感染的[仓鼠肾]BHK细胞中分离到的可溶组抗原是一种相对分子质量为38000的单一蛋白，并与从病毒颗粒中分离的蛋白7在聚丙烯酰胺凝胶上共迁移。通过排除层析和等电聚焦纯化得到一种蛋白质，其抗原性似乎与在病毒收获中发现的天然群抗原相同；它对绵羊不具传染性，被认为在很大程度上不受宿主细胞和其他病毒特定蛋白质的影响。这种抗原显然可以安全地用于诊断程序。
Soluble group antigen isolated from bluetongue virus infected [hamster kidney] BHK cells was shown to be a single protein with a MW of 38,000 and to co-migrate on polyacrylamide gels with protein 7 isolated from virus particles. Purification by exclusion chromatography and isoelectric focusing yields a protein that appears to be antigenically identical to the native group antigen found in virus harvests; it is noninfectious for sheep and is thought to be largely free from host cell and other virus specified proteins. This antigen apparently could be used in diagnostic procedures with safety.