INFLUENCE OF GLYCOSAMINOGLYCANS ON FORMATION OF FIBERS FROM MONOMERIC TROPOCOLLAGEN IN-VITRO
INFLUENCE OF GLYCOSAMINOGLYCANS ON FORMATION OF FIBERS FROM MONOMERIC TROPOCOLLAGEN IN-VITRO
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DOI：
10.1111/j.1432-1033.1973.tb02739.x
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
OBRINK, B
中科院分区：
文献类型：
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作者：
OBRINK, B
在体外胶原纤维形成的比浊法研究中，使用了特征良好的单体乳酸原胶原和各种特征良好的糖胺聚糖制剂。实验在生理离子条件和37°C下进行。其他人已经表明，原纤化过程包括两个阶段，成核阶段和纤维生长阶段。通过在成核阶段之前和之后添加多糖，可以分别区分多糖对成核和对纤维生长的影响。硫酸软骨素、硫酸皮肤素、硫酸乙酰肝素、肝素和硫酸软骨素和硫酸皮肤素的蛋白聚糖在4°C下与原胶原形成复合物，当它们在成核阶段之前加入时，在大多数情况下加速纤维形成。这种现象归因于对成核过程的影响。当在成核阶段硫酸软骨素之后添加时，硫酸乙酰肝素级分，尤其是蛋白聚糖延迟了纤维形成。这一观察结果归因于从核抑制纤维生长。透明质酸通过空间排阻增加原胶原分子的活性，加速成核和纤维生长过程。不与胶原形成复合物的硫酸角质素在成核前或成核后添加时没有或略有减速作用。通过光散射技术可以证明某些糖胺聚糖影响在早期纤维形成过程中形成的胶原纤维的性质。观察到某些多糖对胶原纤维形成具有不同的影响，当在成核之前或之后加入时，表明胶原的聚集（成核）状态在确定多糖所发挥的作用方面是最重要的。这一发现可以解释文献中一些相互矛盾的数据。化学和物理异质性的糖胺聚糖也影响其与胶原蛋白的相互作用模式在纤维形成。
Well‐characterized, monomeric, lathyritic tropocollagen and a variety of well‐characterized glycosaminoglycan preparations have been used in turbidimetric studies on collagen fiber formationin vitro.The experiments have been performed under physiological ionic conditions and at 37°C. It has been shown by others that the fibrillogenetic process consists of two stages, a nucleation phase and a fiber growth phase. By addition of polysaccharides before and after the nucleation phase it was possible to differentiate between the effects of the polysaccharides on the nucleation and on the fiber‐growth, respectively. Chondroitin sulphate, dermatan sulphate, heparan sulphate, heparin and proteoglycans of chondroitin sulphate and dermatan sulphate, which at 4°C form complexes with tropocollagen, in most cases accelerated the fiber formation, when they were added before the nucleation phase. This phenomenon was ascribed to an effect on the nucleation process. When added after the nucleation phase chondroitin sulphate, a heparan sulphate fraction and especially the proteoglycans delayed the fiber formation. This observation was ascribed to an inhibition of fiber growth from the nuclei.Hyaluronic acid, which increases the activity of the tropocollagen molecules by steric exclusion, accelerated both the nucleation and the fiber growth processes. Keratan sulphate, which does not form complexes with collagen, had no or a slightly decelerating effect, either when added before or after the nucleation.It could be demonstrated by light‐scattering technique that certain glycosaminoglycans influenced the properties of the collagen fibrils formed during the early fiber formation process.The observation that certain polysaccharides had different effects on collagen fiber formation, when added before or after the nucleation, shows that the state of aggregation (nucleation) of collagen is of prime importance in determining the effects exerted by the polysaccharides. This finding may explain some of the contradictory data in the literature. Chemical and physical heterogeneity of the glycosaminoglycans also influenced their mode of interaction with collagen during fiber formation.