Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells
Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells
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DOI：
10.1096/fj.09-137174
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发表时间：
2010-07-01
期刊：
影响因子：
4.8
通讯作者：
Heike, Toshio
中科院分区：
文献类型：
--
作者：
Mizuno, Yuta;Chang, Hsi;Heike, Toshio
诱导性多能干细胞（iPS）是一种由重编程体细胞产生的多能干细胞，预期其具有用于以患者为导向的疾病调查、药物筛选、毒性测试和移植治疗的潜力。在这里，我们证明了小鼠iPS细胞具有体外发育成骨骼肌干/祖细胞的潜力，其几乎等同于小鼠胚胎干细胞。使用抗卫星细胞抗体SM/C-2.6通过荧光激活细胞分选有效地富集具有强的体外成肌潜能的细胞。此外，在移植到mdx小鼠中时，SM/C-2.6（+）细胞在受伤肌肉中表现出持续的肌源性谱系分化，同时提供长寿命的肌肉干细胞支持。我们的数据表明，iPS细胞有潜力用于临床治疗肌营养不良症。水野，Y.，张，H.，Umeda，K.，Niwa，A.，Iwasa，T.，Awaya，T.，S.C.，Yamamoto，H.，Yamanaka，S.，Nakahata，T.，Heike，T.从小鼠诱导多能干细胞产生骨骼肌干/祖细胞。FASEB J.24，2245-2253（2010）。www.fasebj.org
Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6(+) cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.-Mizuno, Y., Chang, H., Umeda, K., Niwa, A., Iwasa, T., Awaya, T., Fukada, S., Yamamoto, H., Yamanaka, S., Nakahata, T., Heike, T. Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. FASEB J. 24, 2245-2253 (2010). www.fasebj.org