M-CELL TRANSPORT OF VIBRIO-CHOLERAE FROM THE INTESTINAL LUMEN INTO PEYERS-PATCHES - A MECHANISM FOR ANTIGEN SAMPLING AND FOR MICROIAL TRANS-EPITHELIAL MIGRATION
M-CELL TRANSPORT OF VIBRIO-CHOLERAE FROM THE INTESTINAL LUMEN INTO PEYERS-PATCHES - A MECHANISM FOR ANTIGEN SAMPLING AND FOR MICROIAL TRANS-EPITHELIAL MIGRATION
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DOI：
10.1093/infdis/153.6.1108
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发表时间：
1986-06-01
影响因子：
6.4
通讯作者：
CRAY, WC
中科院分区：
文献类型：
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作者：
OWEN, RL;PIERCE, NF;CRAY, WC
将活的霍乱弧菌 O1 接种到未免疫兔子的肠腔中。弧菌被派尔氏淋巴滤泡上的 M 细胞吞噬，通过上皮被携带在囊泡中，并在下面的淋巴细胞和巨噬细胞中排出。用[2-3H]腺嘌呤标记的霍乱弧菌放射自显影证实了转运。带有和不带有荚膜的本土细菌也从控制环中取出并通过M细胞进入派尔氏淋巴集结。没有采用酸化、福尔马林、加热或紫外线照射杀死的霍乱弧菌，这一结果可能与口服疫苗的开发有关。钌红染色显示 M 细胞上粘液层的间隙、桥接弧菌和 M 细胞微绒毛之间空间的糖萼以及 M 细胞微绒毛膜上的节状突起；在吸收细胞上没有发现这些投影。因此，M 细胞将活的肠道微生物（包括非侵入性的霍乱弧菌）输送到肠道淋巴组织中，在那里启动粘膜免疫反应。 M 细胞的摄取和运输也可能有助于某些病原菌穿过粘膜屏障并引发全身感染。
Viable Vibrio cholerae O1 were inoculated into the intestinal lumen of nonimmune rabbits. The vibrios were phagocytosed by M cells over Peyer''s patch lymphoid follicles, carried in vesicles through the epithelium, and discharged among underlying lymphocytes and macrophages. Autoradiography of V. cholerae labeled with [2-3H]adenine confirmed transport. Indigenous bacteria with and without capsules were also taken up from control loops and carried through M cells into Peyer''s patches. V. cholerae killed by acidification, formalin, heat, or UV irradiation were not taken up, a result that may have relevance for development of oral vaccines. Ruthenium red stain revealed gaps in the layer of mucus over M cells, glycocalyx bridging the space between vibrios and M cell microvilli, and knobby projections over membranes of M cell microvilli; these projections were not found over absorptive cells. M cells thus convey viable enteric microbes, including V. cholerae that are not otherwise invasive, into intestinal lymphoid tissue, where mucosal immune responses are initiated. Uptake and transport by M cells may also assist certain pathogenic bacteria in traversing the mucosal barrier and initiating systemic infection.