Identification of tumor angiogenesis-related genes by subtractive hybridization.
Identification of tumor angiogenesis-related genes by subtractive hybridization.
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通过消减杂交鉴定肿瘤血管生成相关基因。
DOI：
10.1006/mvre.2000.2242
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Nguyen,M
中科院分区：
文献类型：
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作者：
Wang,JL;Liu,YH;Lee,MC;Nguyen,TM;Lee,C;Kim,A;Nguyen,M
实体瘤的生长和转移依赖于它们启动和维持新的毛细血管生长的能力，即血管生成（Folkman, 1995）。尽管关于成人血管生成已经发现了很多，但目前尚不清楚发生在实体瘤生长中的异常血管生成与发生在子宫内膜增殖或伤口愈合中的理想血管生成是否涉及不同的机制。过去，许多研究人员通过抗体靶向（Huang et al., 1997）和已知内皮粘附分子的免疫组织化学分析（Nguyen et al., 1997）等多种方法研究了增殖性肿瘤内皮与正常静止内皮之间的差异。我们进一步研究了肿瘤血管生成的分子机制，通过鉴定当静止内皮细胞暴露于肿瘤环境时激活和下调的基因。为了实现这一目标，我们使用了一种称为SSH （suppression subtractive hybridization, Diatchenko et al., 1996）的减法杂交方法。
The growth and metastasis of solid tumors is dependent on their ability to initiate and sustain new capillary growth, ie, angiogenesis (Folkman, 1995). Although much has been discovered about adult angiogenesis, it is unclear whether abnormal angiogenesis such as that occurring in solid tumor growth involves different mechanisms from desirable angiogenesis which occurs in endometrial proliferation or in wound healing. In the past, many researchers have investigated the difference between the proliferating tumor endothelium from the normal quiescent endothelium by many approaches including antibody targeting (Huang et al., 1997) and immunohistochemical analysis of known endothelial adhesion molecules (Nguyen et al., 1997). We further investigated the molecular mechanisms of tumor angiogenesis by identifying genes that become activated as well as those that become down-regulated when quiescent endothelial cells are exposed to a tumor environment. Toward this goal, we used a subtraction hybridization method called SSH (suppression subtractive hybridization, Diatchenko et al., 1996).