Alterations in urinary bladder M2-muscarinic receptor protein and mRNA in 2-week streptozotocin-induced diabetic rats
Alterations in urinary bladder M2-muscarinic receptor protein and mRNA in 2-week streptozotocin-induced diabetic rats
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DOI：
10.1016/s0304-3940(99)00871-x
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发表时间：
1999-12-31
影响因子：
2.5
通讯作者：
Cheng, JT
中科院分区：
文献类型：
--
作者：
Tong, YC;Chin, WT;Cheng, JT
M-2受体（M-2- machr）是动物膀胱中占优势的毒蕈碱亚型。研究了糖尿病性膀胱病变过程中其蛋白含量和生物合成的变化。将3月龄雄性Wistar大鼠分为两组：(1)2周龄糖尿病大鼠；(2)血糖控制正常大鼠。单次静脉注射链脲佐菌素60 mg/kg诱导糖尿病。单克隆抗体Western免疫印迹法检测大鼠膀胱体组织中M-2受体蛋白的含量。采用Northern blotting法测定膀胱组织中Mp毒蕈碱受体mRNA的含量。Western免疫印迹结果显示，与对照组相比，糖尿病膀胱中M-2-mAChR蛋白含量显著升高40.0 +/- 6.2% （P < 0.05, n = 8）。Northern blotting结果显示，糖尿病膀胱M-2-mAChR mRNA表达增加69.3 +/- 8.5% （P < 0.05, n = 8）。本研究结果表明糖尿病膀胱中M-2-mAChR生物合成上调。这种现象可能导致对乙酰胆碱的反应性增加，从而导致逼尿肌不稳定。1999爱思唯尔科学爱尔兰有限公司版权所有。
The M-2 receptor (M-2-mAChR) is quantitatively the dominant muscarinic subtype in animal bladders. The alterations in its protein quantity and biosynthesis during diabetic cystopathy were investigated. Three-month-old male Wistar rats were divided into two groups: (1) 2-week-old diabetics; and (2) normoglycemic control rats. Diabetes was induced by single intravenous injection of 60 mg/kg streptozotocin. The amount of M-2 receptor protein in the rat bladder body tissue was measured by Western immunoblotting using monoclonal antibodies. For determination of Mp muscarinic receptor mRNA in the bladder tissue, the method of Northern blotting was employed. The results of the Western immunoblotting showed that the amount of M-2-mAChR protein in the diabetic bladder was significantly increased by 40.0 +/- 6.2% when compared with the control bladder (P < 0.05, n = 8). The Northern blotting demonstrated a 69.3 +/- 8.5% increase of the M-2-mAChR mRNA in the diabetic bladder (P < 0.05, n = 8). The findings of the present study demonstrated an upregulation of M-2-mAChR biosynthesis in the diabetic urinary bladder. This phenomenon could lead to increased reactivity to acetylcholine and thus results in detrusor instability. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.