Genome-Wide Detection of Predicted Non-coding RNAs Related to the Adhesion Process in Vibrio alginolyticus Using High-Throughput Sequencing.
Genome-Wide Detection of Predicted Non-coding RNAs Related to the Adhesion Process in Vibrio alginolyticus Using High-Throughput Sequencing.
复制标题
使用高通量测序对与溶藻弧菌粘附过程相关的预测非编码 RNA 进行全基因组检测
DOI：
10.3389/fmicb.2016.00619
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发表时间：
2016
影响因子：
5.2
通讯作者：
Yan Q
中科院分区：
文献类型：
--
作者：
Huang L;Hu J;Su Y;Qin Y;Kong W;Zhao L;Ma Y;Xu X;Lin M;Zheng J;Yan Q
细菌粘附鱼粘液的能力可受环境条件的影响，被认为是溶藻弧菌的关键毒力因子。然而，这种能力背后的分子机制仍不清楚。我们以前的研究表明，压力条件，如暴露于铜，铅，汞，和低pH值能够降低溶藻弧菌的粘附能力。非编码RNA（ncRNA）在细菌基因表达的复杂调控中起着至关重要的作用，从而影响细菌的致病性。因此，我们假设ncRNA在溶藻弧菌粘附过程中起关键作用。为了验证这一点，我们将高通量测序与计算技术相结合，以检测应激处理后样品中的ncRNA动态。通过QPCR证实随机选择的新ncRNA的表达。在显著改变的ncRNA中，30个被所有胁迫处理上调，2个下调。QPCR结果加强了测序数据的可靠性。靶点预测和KEGG途径分析表明，这些ncRNA与体外粘附相关的途径密切相关，我们的结果表明化学应激诱导的溶藻弧菌粘附能力的降低可能是由于ncRNA表达的扰动。我们的研究结果为进一步研究溶藻弧菌黏附过程中ncRNA的功能提供了重要信息。
The ability of bacteria to adhere to fish mucus can be affected by environmental conditions and is considered to be a key virulence factor of Vibrio alginolyticus. However, the molecular mechanism underlying this ability remains unclear. Our previous study showed that stress conditions such as exposure to Cu, Pb, Hg, and low pH are capable of reducing the adhesion ability of V. alginolyticus. Non-coding RNAs (ncRNAs) play a crucial role in the intricate regulation of bacterial gene expression, thereby affecting bacterial pathogenicity. Thus, we hypothesized that ncRNAs play a key role in the V. alginolyticus adhesion process. To validate this, we combined high-throughput sequencing with computational techniques to detect ncRNA dynamics in samples after stress treatments. The expression of randomly selected novel ncRNAs was confirmed by QPCR. Among the significantly altered ncRNAs, 30 were up-regulated and 2 down-regulated by all stress treatments. The QPCR results reinforced the reliability of the sequencing data. Target prediction and KEGG pathway analysis indicated that these ncRNAs are closely related to pathways associated with in vitro adhesion, and our results indicated that chemical stress-induced reductions in the adhesion ability of V. alginolyticus might be due to the perturbation of ncRNA expression. Our findings provide important information for further functional characterization of ncRNAs during the adhesion process of V. alginolyticus.