Laminin α1 chain G domain peptide, RKRLQVQLSIRT, inhibits epithelial branching morphogenesis of cultured embryonic mouse submandibular gland
Laminin α1 chain G domain peptide, RKRLQVQLSIRT, inhibits epithelial branching morphogenesis of cultured embryonic mouse submandibular gland
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层粘连蛋白 α1 链 G 结构域肽 RKRLQVQLSIRT 抑制培养胚胎小鼠颌下腺上皮分支形态发生
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Yoshihiko Yamada
中科院分区：
文献类型：
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作者：
Y. Kadoya;M. Nomizu;L. Sorokin;S. Yamashina;Yoshihiko Yamada
层粘连蛋白α1和α2链羧基末端球状结构域(G域)的活性序列已经通过在一些生物分析中筛选重叠的合成肽来鉴定(Nomizu等人)。[1995]J.Biol.化学。270：20583-20590；野水等人。[1996]FEBS Lett.396：37-42)。我们测试了这些多肽在胚胎第13天小鼠颌下腺外植体中的活性，以确定与器官发育有关的功能部位。层粘连蛋白α1链肽RKRLQVQLSIRT(残基2719-2730，命名为AG-73)显著抑制上皮分支形态发生。相反，包括AASIKVAVSADR和NRWHSIYITRFG在内的其他细胞粘附层粘连蛋白α1链肽不能抑制分支。层粘连蛋白α2链AG-73的同源物MG-73不抑制这种分支。α2链肽没有作用，这可能是由于第13天小鼠层粘连蛋白链水平较低所致。层粘连蛋白α2链特异性单抗与发育成熟的腺泡基底膜呈强阳性反应，但仅与胚胎第13天的颌下上皮呈弱染色。免疫荧光法检测，AG-73和对照杂乱多肽处理的上皮细胞E-钙粘素和α-6整合素的表达均无明显变化。Nidogen/entactin免疫组织化学染色显示，AG-73处理3d的外植体基底膜不连续。用对照多肽处理3d的外植体基底膜正常。这些结果表明，包含层粘连蛋白α1链AG-73序列的区域对于颌下腺在胚胎早期的发育是至关重要的。AG-73处理的外植体中不连续的基底膜可能表明该区域在基底膜组装中起着重要作用。戴夫。戴恩。1998年；212：394-402。©1998 Wiley-Liss，Inc.
Active sequences from the laminin α1 and α2 chain carboxyl‐terminal globular domains (G domain) have been identified by screening overlapping synthetic peptides in a number of biological assays (Nomizu et al. [1995] J. Biol. Chem. 270:20583–20590; Nomizu et al. [1996] FEBS Lett. 396:37–42). We have tested the activity of these peptides in submandibular gland explants of embryonic day 13 mice to determine the functional sites involved in organ development. The laminin α1 chain peptide, RKRLQVQLSIRT (residues 2719–2730 and designated AG‐73), significantly inhibited epithelial branching morphogenesis. In contrast, other cell adhesive laminin α1 chain peptides including the AASIKVAVSADR and NRWHSIYITRFG failed to inhibit the branching. MG‐73, a homologue of AG‐73 from the laminin α2 chain, did not inhibit the branching. The α2 chain peptide had no effect, which may be due to the low levels of this laminin chain in day 13 mice. Laminin α2 chain‐specific monoclonal antibodies strongly reacted with the basement membranes of developed acini but only weakly stained embryonic day 13 submandibular epithelium. The expression of E‐cadherin and α6 integrin, as detected by immunofluorescence, were unchanged in both AG‐73 and control scramble peptide‐treated epithelial cells of the explants. In contrast, immunostaining of nidogen/entactin showed that explants treated with AG‐73 for 3 days had a discontinuous basement membrane. Explants treated for 3 days with control peptide showed a normal basement membrane. These results suggest that the region containing the AG‐73 sequence of the laminin α1 chain is crucial for development of submandibular gland at early embryonic stages. The discontinuous basement membrane in AG‐73‐treated explants may indicate an important role for this region in basement membrane assembly. Dev. Dyn. 1998;212:394–402. © 1998 Wiley‐Liss, Inc.
合成肽竞争性抑制与成纤维细胞的直接结合以及纤连蛋白纯化细胞结合域的功能性生物测定。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Akiyama,SK;Yamada,KM
通讯作者：
Yamada,KM
重组层粘连蛋白 G 结构域介导成肌细胞粘附和肝素结合。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Yurchenco,PD;Sung,U;Ward,MD;Yamada,Y;O'Rear,JJ
通讯作者：
O'Rear,JJ
影响因子：
2.7
作者：
Dong, Qiang;Yuan, Guoqiang;Pan, Yawen
通讯作者：
Pan, Yawen