Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II.
Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II.
复制标题
作者：
白细胞的再循环对于适当的免疫应答是必不可少的。然而，调节白细胞进入细胞的分子机制仍不清楚。在这里，我们表明，plexin-A1，第三类和第六类信号蛋白的主要受体成分，是至关重要的参与树突状细胞（DC）进入神经系统。此外，我们表明，Sema 3A，而不是Sema 6C或Sema 6D，是DC的迁移所需的，并且Sema 3A产生的促炎细胞促进肌动球蛋白收缩在迁移的DC的后缘。这些发现不仅证明了脑信号蛋白信号参与了DC的运输，而且还提供了一种新的机制，当这些细胞通过狭窄的间隙时，诱导肌动球蛋白收缩。
Recirculation of leukocytes is essential for proper immune responses. However, the molecular mechanisms that regulate leukocyte entry into the lymphatics remain unclear. Here we show that plexin-A1, a primary receptor component for class III and class VI semaphorins, is crucially involved in the entry of dendritic cells (DCs) into the lymphatics. Additionally, we show that Sema3A, but not Sema6C or Sema6D, is required for DC transmigration, and that Sema3A produced by the lymphatics promotes actomyosin contraction at the trailing edge of migrating DCs. These findings not only demonstrate that semaphorin-signals are involved in DC trafficking but also provide a novel mechanism that induces actomyosin contraction as these cells pass through narrow gaps.