Engineering high coenzyme Q10 tomato
Engineering high coenzyme Q10 tomato
复制标题
工程高辅酶Q10番茄。
DOI：
10.1016/j.ymben.2021.09.007
复制
发表时间：
2021-09-24
影响因子：
8.4
通讯作者：
Xu, Jing-Jing
中科院分区：
文献类型：
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作者：
Fan, Hang;Liu, Yan;Xu, Jing-Jing
辅酶Q（CoQ）是生物体能量代谢的关键。在人类中，辅酶Q10缺乏会导致疾病，必须通过饮食补充;然而，植物性食物中的辅酶Q含量主要较低。在这里，我们报告了高辅酶Q10番茄品系的育种表达四种酶与水果特异性启动子，它修改叶绿体分支酸途径，增强胞质类异戊二烯生物合成，并上调前两个反应，构建辅酶Q10 polyisoprenoid尾巴。我们表明，虽然芳香族前体的水平可以显着提高，头基异戊二烯化是提高最终辅酶Q10产量的关键。在表达所有四种转基因的HUCD品系中，最高CoQ 10含量（0.15 mg/g干重）显示出比野生型水平增加了7倍，并达到了非常丰富的CoQ 10食品级。通过转录组和代谢分析概述其他萜类化合物的变化，揭示了HUCD系中类胡萝卜素和α-生育酚的可变含量。除了不同萜类途径之间的神秘关系外，还可以选择保持大量维生素水平的高辅酶Q10植物。我们的研究为开发富含辅酶Q10的作物作为膳食补充剂铺平了道路。
Coenzyme Q (CoQ) is vital for energy metabolism in living organisms. In humans, CoQ10 deficiency causes diseases and must be replenished via diet; however, CoQ content in plant foods is primarily low. Here, we report the breeding of high CoQ10 tomato lines by expressing four enzymes with a fruit-specific promoter, which modifies the chloroplast chorismate pathway, enhances cytosolic isoprenoid biosynthesis, and up-regulates the first two reactions in mitochondrion that construct the CoQ10 polyisoprenoid tail. We show that, while the level of the aromatic precursor could be markedly elevated, head group prenylation is the key to increasing the final CoQ10 yield. In the HUCD lines expressing all four transgenes, the highest CoQ10 content (0.15 mg/g dry weight) shows a seven-fold increase from the wild-type level and reaches an extraordinarily rich CoQ10 food grade. Overviewing the changes in other terpenoids by transcriptome and metabolic analyses reveals variable contents of carotenoids and alpha-tocopherol in the HUCD lines. In addition to the enigmatic relations among different terpenoid pathways, high CoQ10 plants maintaining substantial levels of either vitamin can be selected. Our investigation paves the way for the development of CoQ10-enriched crops as dietary supplements.