Membrane-associated protein kinase activities in the developing mesocarp of grape berry
Membrane-associated protein kinase activities in the developing mesocarp of grape berry
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DOI：
10.1078/0176-1617-01038
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发表时间：
2004-01-01
影响因子：
4.3
通讯作者：
Zhang, DP
中科院分区：
文献类型：
--
作者：
Shen, YY;Duan, CQ;Zhang, DP
果实发育是一个涉及各种信号和基因表达的过程，已知蛋白激酶催化的蛋白质磷酸化在真核细胞信号传导中发挥关键作用，因此可能参与果实发育的调控。利用外源底物磷酸化的方法，我们表征了钙依赖性和钙调蛋白非依赖性蛋白激酶（CDPK）活性和髓磷脂碱性蛋白（MBP）磷酸化活性，这可能是由于葡萄浆果发育中果皮中丝裂原激活蛋白激酶（MAPK）样活性所致。 CDPK 活性主要位于质膜中，而 MAPK 样活性主要与内膜相关。二价阳离子需求量测定表明，Mn2+可以在一定程度上替代孵育体系中的Mg2+发挥蛋白激酶活性。 CDPK 和 MAPK 样活性均耐受热处理。这两种酶的活性具有果实发育阶段特异性，两种酶的活性在成熟阶段之前的滞后生长阶段最高，强烈表明检测到的 CDPK 和 MAPK 样活性在果实发育中的重要作用。
Fruit development is a process involving various signals and gene expression, Protein phosphorylation catalyzed by protein kinases is known to play a key role in eukaryotic cell signalling and so may be involved in the regulation of fruit development. Using the method of exogenous substrate phosphorylation, we characterised the calcium-de pendent and calmodulin-independent protein kinase (CDPK) activity and the myelin basic protein (MBP)-phosphoralating activity that could be due to a mitogen-activated protein kinase (MAPK)-like activity in the developing mesocarp of grape berry. The CDPK activity was shown to be predominantly localised in the plasma membrane, while the MAPK-like activity was predominantly associated with endomembranes. The assays of bivalent cation requirement showed that Mn2+ could to a certain extent replace Mg2+ in the incubation system for the protein kinase activities. Both CDPK and MAPK-like activities were resistant to heat treatment. The activities of the two enzymes were fruit developmental stage-specific with the highest activities of both enzymes in the lag growth phase before the ripening stage, suggesting strongly the important roles of the detected CDPK and MAPK-like activities in the fruit development.