RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors: an immunohistochemical study of 131 cases
RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors: an immunohistochemical study of 131 cases
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DOI：
10.1038/modpathol.2010.195
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发表时间：
2011-02-01
期刊：
影响因子：
7.5
通讯作者：
Li, Jianping
中科院分区：
文献类型：
--
作者：
Cao, Dengfeng;Liu, Aijun;Li, Jianping
LIN 28在小鼠原始生殖细胞发育和生殖细胞恶性转化中具有重要作用。在这项研究中，我们检测了131例原发性人类性腺外生殖细胞肿瘤中LIN 28的免疫组化谱（中枢神经系统（CNS）76例，纵隔17例，骶尾部30例，骨盆3例，阴道2例，肝脏1例，网膜1例，腹膜后1例），包括以下肿瘤和/或成分：57例卵巢癌/生殖细胞瘤、10例胚胎癌、74例卵黄囊瘤、6例绒毛膜癌、15例成熟畸胎瘤和13例未成熟畸胎瘤。我们将LIN 28与SALL 4进行比较，以评估其诊断价值。为了确定其特异性，我们在406例性腺外非生殖细胞肿瘤（103例癌，91例肉瘤，9例黑色素瘤，12例间皮瘤，83例淋巴瘤，9例浆细胞瘤，82例CNS肿瘤和17例胸腺上皮肿瘤）中检测了LIN 28。染色半定量评分为0（无细胞染色）、1+（0-30%）、2+（31-60%）、3+（61-90%）和4+（>90%）。LIN 28染色见于所有卵巢癌/生殖细胞瘤（1例3+，56例4+）、胚胎癌（所有10例4+）和卵黄囊肿瘤（3例3+，71例4+）。在6例绒毛膜癌中的5例（1+至4+）、13例未成熟畸胎瘤中的8例（未成熟成分中的1+至2+）和15例成熟畸胎瘤中的1例（1+）中观察到不同的LIN 28染色。406例非生殖细胞肿瘤中仅11例显示1+ LIN 28染色。因此，LIN 28是原发性性腺外腺瘤/生殖细胞瘤、胚胎癌和卵黄囊肿瘤的敏感（100%敏感性）标记物，具有高特异性。与SALL 4相比，LIN 28对乳腺癌/生殖细胞瘤和胚胎癌的诊断敏感性水平相似。对于原发性性腺外卵黄囊肿瘤，尽管SALL 4染色所有肿瘤（1例1+，2例2+，10例3+，61例4+），但LIN 28染色更多肿瘤细胞（平均95 vs 90%，P - 0.03），因此更敏感。对于原发性性腺外卵黄囊肿瘤，LIN 28和SALL 4的组合可以实现比单独使用更高的诊断灵敏度。现代病理学（2011）24，288-296; doi：10.1038/modpathol.2010.195;在线发表2010年11月5日
LIN28 has been shown to have an important role in primordial germ cell development and malignant transformation of germ cells in mouse. In this study, we examined the immunohistochemical profile of LIN28 in 131 primary human extragonadal germ cell tumors (central nervous system (CNS) 76, mediastinum 17, sacrococcygeal region 30, pelvis 3, vagina 2, liver 1, omentum 1, and retroperitoneum 1), including the following tumors and/or components: 57 seminomas/germinomas, 10 embryonal carcinomas, 74 yolk sac tumors, 6 choriocarcinomas, 15 mature, and 13 immature teratomas. We compared LIN28 with SALL4 to assess its diagnostic value. To determine its specificity, we examined LIN28 in 406 extragonadal-non-germ cell tumors (103 carcinomas, 91 sarcomas, 9 melanomas, 12 mesotheliomas, 83 lymphomas, 9 plasmacytomas, 82 CNS tumors, and 17 thymic epithelial tumors). The staining was semi-quantitatively scored as 0 (no cell stained), 1+ (0-30%), 2+ (31-60%), 3+ (61-90%), and 4+ (>90%). LIN28 staining was seen in all seminomas/germinomas (3+ in 1 and 4+ in 56), embryonal carcinomas (4+ in all 10), and yolk sac tumors (3+ in 3 and 4+ in 71). Variable LIN28 staining was seen in 5 of 6 choriocarcinomas (1+ to 4+), 8 of 13 immature teratomas (1+ to 2+ in immature elements), and in 1 of 15 mature teratomas (1+). Only 11 of 406 non-germ cell tumors showed 1+ LIN28 staining. Therefore, LIN28 is a sensitive (100% sensitivity) marker for primary extragonadal seminomas/germinomas, embryonal carcinomas, and yolk sac tumors with high specificity. Compared with SALL4, LIN28 demonstrated a similar level of diagnostic sensitivity for seminomas/germinomas and embryonal carcinomas. For primary extragonadal yolk sac tumors, although SALL4 stained all tumors (1+ in 1, 2+ in 2, 3+ in 10, and 4+ in 61), LIN28 stained more tumor cells (mean 95 vs 90%, P - 0.03) and was therefore more sensitive. For primary extragonadal yolk sac tumors, combining LIN28 and SALL4 can achieve a higher diagnostic sensitivity than either alone. Modern Pathology (2011) 24, 288-296; doi:10.1038/modpathol.2010.195; published online 5 November 2010