Effects of Normothermic Conditioned Microwave Irradiation on Cultured Cells Using an Irradiation System with Semiconductor Oscillator and Thermo-regulatory Applicator.
Effects of Normothermic Conditioned Microwave Irradiation on Cultured Cells Using an Irradiation System with Semiconductor Oscillator and Thermo-regulatory Applicator.
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DOI：
10.1038/srep41244
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发表时间：
2017-02-01
影响因子：
4.6
通讯作者：
Tabuse K
中科院分区：
文献类型：
--
作者：
Asano M;Sakaguchi M;Tanaka S;Kashimura K;Mitani T;Kawase M;Matsumura H;Yamaguchi T;Fujita Y;Tabuse K
我们研究了常温条件下微波辐射对培养细胞的影响。为此，我们研制了一套由半导体微波振荡器（2.45GHz）和调温辐射器组成的辐射系统，该系统能以不同的输出功率辐射微波，使培养细胞的温度保持在37 °C。八种培养细胞中有七种被微波辐射杀死，其中四种不受42.5 °C热处理的影响。由于在2.45 GHz下，细胞类型和介质之间的介电性质如ε '、ε”和tanδ显示出相似的值，因此细胞吸收微波能量的程度在细胞类型之间可能几乎相同。因此，细胞对微波辐射的脆弱性在细胞类型之间可能是不同的。在HL-60细胞，这是最敏感的微波照射，随着照射时间和照射输出的增加，生存能力下降;相应地，在生存能力的下降与总焦耳的增加。然而，当每分钟提供高或低的焦耳量时，细胞活力和总焦耳之间的相关性变得相对较弱。假设在常温细胞条件下，各种癌细胞被各自的特定微波输出有效地杀死。
We investigated the effects of microwave irradiation under normothermic conditions on cultured cells. For this study, we developed an irradiation system constituted with semiconductor microwave oscillator (2.45 GHz) and thermos-regulatory applicator, which could irradiate microwaves at varied output powers to maintain the temperature of cultured cells at 37 °C. Seven out of eight types of cultured cells were killed by microwave irradiation, where four were not affected by thermal treatment at 42.5 °C. Since the dielectric properties such as ε’, ε” and tanδ showed similar values at 2.45 GHz among cell types and media, the degree of microwave energy absorbed by cells might be almost the same among cell types. Thus, the vulnerability of cells to microwave irradiation might be different among cell types. In HL-60 cells, which were the most sensitive to microwave irradiation, the viability decreased as irradiation time and irradiation output increased; accordingly, the decrease in viability was correlated to an increase in total joule. However, when a high or low amount of joules per minute was supplied, the correlation between cellular viability and total joules became relatively weak. It is hypothesized that kinds of cancer cells are efficiently killed by respective specific output of microwave under normothermic cellular conditions.